R-Biopharm AG, An der neuen Bergstraße 17, Darmstadt 64297, Germany.
ChemStats Consulting, Minneapolis, MN, USA.
J AOAC Int. 2023 Mar 1;106(2):341-347. doi: 10.1093/jaoacint/qsac145.
Enzytec™ Liquid Ethanol was approved as AOAC Official MethodSM2017.07 First Action in September 2017 and is now further characterized by a collaborative study using the manual and automated version of the test.
It is applicable to quantify ethanol in diluted or undiluted kombucha, fruit juices, vegetable juices, and alcohol-free beer samples around 0.5% ABV within 20 min using two ready-to-use reagents and measurement of absorbance at 340 nm.
The overall relative reproducibility standard deviation across a wide concentration range for kombucha was calculated to be 6.99% by modeling the reproducibility standard deviation by the mean concentration for each of the six kombucha pairs by a linear regression. Analysis of juices and beer showed an overall higher variation with an estimated overall RSD(R) value by regression of 10.1%. Mean recovery of aqueous ethanol reference solutions tested by each participant was between 100 and 103%.
The data obtained by this collaborative study show that the EnzytecTMLiquid Ethanol is suitable to quantify ethanol from matrices representing important alcohol-free liquid food categories.
The EnzytecTMLiquid Ethanol was approved as AOAC Method 2017.07 Final Action.
Enzytec™ 液体乙醇于 2017 年 9 月被批准为 AOAC 官方方法 SM2017.07 第一行动，现在通过使用手动和自动测试版本的协作研究进一步进行了特征描述。
该方法适用于在 20 分钟内定量测定 ABV 约为 0.5%的康普茶、果汁、蔬菜汁和无酒精啤酒样品中的乙醇，使用两种即用型试剂并测量 340nm 处的吸光度。
通过对 6 对康普茶中每个康普茶的平均浓度进行线性回归，计算出整个宽浓度范围内康普茶再现性标准偏差的总体相对再现性标准偏差为 6.99%。对果汁和啤酒的分析显示，整体变化较大，通过回归估计的整体 RSD(R)值为 10.1%。每个参与者测试的水乙醇参考溶液的平均回收率在 100%至 103%之间。
通过这项协作研究获得的数据表明，EnzytecTMLiquid Ethanol 适合于从代表重要无酒精液体食品类别的基质中定量测定乙醇。
EnzytecTMLiquid Ethanol 被批准为 AOAC 方法 2017.07 最终行动。
