Key Laboratory of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
Anal Chem. 2022 Nov 22;94(46):16265-16273. doi: 10.1021/acs.analchem.2c04401. Epub 2022 Nov 11.
Inspired by the headspace sampling (HS) device, a versatile high-throughput photochemical reactor with twenty vessels serving as both the photochemical vapor generator and the HS device was developed for the rapid and sensitive determination of mercury, nickel, and selenium by coupling photochemical vapor generation (PVG) to atomic fluorescence spectrometry (AFS) or point discharge optical emission spectrometry (μPD-OES). The photochemical reactor utilized a specially designed annular UV lamp around which the vessels containing sample solution were automatically rotated and irradiated to yield gaseous analyte species. Subsequently, the species escaped into the headspace of vessels prior to introduction to the atomic spectrometer. Compared with the conventional flow injection (FI) or continuous flow (CF) PVG, the developed PVG-HS method possesses several unique advantages including high throughput (260 pcs h), high sensitivity, and the elimination of matrix interference from transition metal ions and the memory effect associated with the quantification of mercury. Limits of detection (LODs) of 0.002, 0.007, and 0.01 μg L were obtained for Hg (II), Ni (II), and Se (IV) by PVG-HS-AFS, respectively, and 0.02 and 0.2 μg L were obtained for Hg (II) and Ni (II) by PVG-HS-μPD-OES, respectively. The practicality of the reactor was evaluated by the detection of Hg (II), Ni (II), and Se (IV) in five certified reference materials, including water (GBW08603, GBW08607, and GBW(E)080395), National Research Council Canada dogfish liver (DOLT-5), fish protein (DORM-4), and three river water samples with good recoveries (92-106%).
受头部空间采样 (HS) 装置的启发，开发了一种多功能高通量光化学反应器，该反应器有 20 个容器，既可用作光化学蒸汽发生器，也可用作 HS 装置，用于通过光化学蒸汽发生 (PVG) 与原子荧光光谱法 (AFS) 或放电光学发射光谱法 (μPD-OES) 快速灵敏地测定汞、镍和硒。光化学反应器利用特殊设计的环形 UV 灯，容器中含有样品溶液，容器自动旋转并照射，生成气态分析物。随后，这些物质在引入原子光谱仪之前逸入容器的头部空间。与传统的流动注射 (FI) 或连续流动 (CF) PVG 相比，所开发的 PVG-HS 方法具有几个独特的优势，包括高通量（260 个 h）、高灵敏度以及消除过渡金属离子的基质干扰和与汞定量相关的记忆效应。通过 PVG-HS-AFS 分别获得了 Hg(II)、Ni(II)和 Se(IV)的检出限 (LOD) 为 0.002、0.007 和 0.01 μg L，通过 PVG-HS-μPD-OES 分别获得了 Hg(II)和 Ni(II)的检出限为 0.02 和 0.2 μg L。通过对五个认证参考物质（包括水（GBW08603、GBW08607 和 GBW(E)080395）、加拿大国家研究委员会狗鱼肝脏（DOLT-5）、鱼蛋白（DORM-4）和三个河水样品）中 Hg(II)、Ni(II)和 Se(IV)的检测，评估了该反应器的实用性，回收率良好（92-106%）。
