School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, 050018, PR China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, PR China.
School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, 050018, PR China.
Biosens Bioelectron. 2023 Jan 15;220:114879. doi: 10.1016/j.bios.2022.114879. Epub 2022 Nov 4.
Circulating microRNAs (miRNAs) can be regarded as reliable noninvasive biomarkers in body fluids for the early diagnosis, prognosis, and monitoring of cancers. By combining target triggered bipedal DNAzyme walker cleavage cycling amplification and planar intercalated methylene blue (MB) molecules amplification, a versatile ratiometric electrochemical biosensing system is constructed for miRNAs detection. Using the microRNA-21 (miRNA-21) as a triggered model target from breast cancer cells (MCF-7) and cervical cancer cells (HeLa), the sensitivity and feasibility of the ratiometric biosensing strategy were verified on the basis of decreased streptavidin-conjugated cupric sulfide@platinum (CuS@Pt-SA) nanozyme signal with cleaved Zn-dependent DNAzyme walkers as well as enhanced duplex section of MB signal, which were assisted by the modification of high electronic conductivity and specific surface area of metallic WSe nanoflowers on the electrode. Hence, the introduced sensing strategy of higher cleavage activity of the bipedal DNAzyme walker cyclic amplification resulted into the remarkable sensitive measurement which had a detection limit of 0.16 fM from 1 fM to 1 nM for miRNA-21. Benefiting from the precise design of the capture Hairpin DNA, this proposed method showed excellent specificity to distinguish miRNA-21 from other miRNAs sequences, in addition to possessing good stability and reproducibility. Thus, this versatility platform can be utilized to sense various miRNAs biomarkers by simple of the redesigning the capture Hairpin DNA, hence presents a great promise in clinical application towards early cancer diagnosis, biological analysis and prognosis.
循环 microRNAs (miRNAs) 可以被视为体液中用于癌症早期诊断、预后和监测的可靠非侵入性生物标志物。通过结合目标触发双足 DNA 酶 walker 切割循环扩增和平面嵌入亚甲基蓝 (MB) 分子扩增，构建了一种用于 miRNA 检测的多功能比率电化学生物传感系统。使用 microRNA-21 (miRNA-21) 作为来自乳腺癌细胞 (MCF-7) 和宫颈癌细胞 (HeLa) 的触发模型靶标，基于减少与切割 Zn 依赖性 DNA 酶 walker 结合的链霉亲和素缀合的硫化铜@铂 (CuS@Pt-SA) 纳米酶信号以及增强 MB 信号的双链部分，验证了比率生物传感策略的灵敏度和可行性，这是通过修饰电极上的高导电性和比表面积的金属 WSe 纳米花辅助实现的。因此，引入的双足 DNA 酶 walker 循环扩增具有更高切割活性的传感策略导致了显著灵敏的测量，对于 miRNA-21，其检测限从 1 fM 到 1 nM 为 0.16 fM。受益于捕获发夹 DNA 的精确设计，该方法除了具有良好的稳定性和重现性外，还具有优异的特异性，可以区分 miRNA-21 与其他 miRNA 序列。因此，这种多功能平台可以通过简单的重新设计捕获发夹 DNA 来用于感应各种 miRNA 生物标志物，因此在临床应用中具有很大的早期癌症诊断、生物分析和预后的应用前景。
