School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
No. 6 Traditional Chinese Medicine Factory, Tianjin Zhongxin Pharmaceutical Group Corporation Ltd, Tianjin, China.
Brain Res. 2023 Jan 1;1798:148158. doi: 10.1016/j.brainres.2022.148158. Epub 2022 Nov 8.
Chronic cerebral hypoperfusion (CCH) is a major risk factor for cognitive decline and degenerative processes. Shunaoxin dropping pill (SNX) has been clinically used to treat cerebrovascular diseases. However, the effect and mechanism of SNX in treating CCH-induced cognitive impairment remain unclear. In this study, CCH was induced in rats using permanent bilateral common carotid artery ligation (2-VO). CCH rats were characterized by impaired spatial learning and memory ability, as well as increased oxidative stress and inflammation in the hippocampus. Additionally, CCH rats had reduced richness and biodiversity of fecal microbiota, which showed a strong correlation with altered serum metabolites. SNX significantly improved the cognitive impairment and restored the dysbiosis of fecal microbiota and serum metabolites in CCH rats. Notably, SNX did not prevent cognitive impairment in antibiotics-treated CCH rats. Our findings suggest that the microbiota-gut-brain axis is a promising therapeutic target for the treatment of CCH-induced cognitive impairment.
慢性脑灌注不足（CCH）是认知能力下降和退行性过程的主要危险因素。参乌心脑通滴丸（SNX）已在临床上用于治疗脑血管疾病。然而，SNX 治疗 CCH 诱导的认知障碍的效果和机制仍不清楚。在这项研究中，使用永久性双侧颈总动脉结扎（2-VO）在大鼠中诱导 CCH。CCH 大鼠的特征是空间学习和记忆能力受损，以及海马体氧化应激和炎症增加。此外，CCH 大鼠粪便微生物群的丰富度和生物多样性减少，这与血清代谢物的改变有很强的相关性。SNX 显著改善了 CCH 大鼠的认知障碍，并恢复了粪便微生物群和血清代谢物的失调。值得注意的是，SNX 不能预防抗生素治疗的 CCH 大鼠的认知障碍。我们的研究结果表明，微生物群-肠道-大脑轴是治疗 CCH 诱导的认知障碍的有前途的治疗靶点。
