Department of Chemistry, Asahi University School of Dentistry, Gifu, Japan.
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Methods Mol Biol. 2023;2582:23-29. doi: 10.1007/978-1-0716-2744-0_3.
The method of labeling proteins of interest with fluorescent dyes that can specifically stain organelles in living cells provides a tool for investigating various cellular processes under a microscope. Visualization (imaging) of the cells using fluorescence has many advantages, including the ability to stain multiple cell organelles and intracellular proteins simultaneously and discriminately, and is used in many research fields. In this chapter, we describe the observation of cell organelles using fluorescence staining to analyze the functions of CCN family proteins involved in various cellular events.
用荧光染料标记感兴趣的蛋白质的方法可以特异性地染色活细胞中的细胞器，为在显微镜下研究各种细胞过程提供了一种工具。使用荧光进行细胞可视化（成像）具有许多优点，包括能够同时且有区别地染色多个细胞细胞器和细胞内蛋白质的能力，并被用于许多研究领域。在本章中，我们描述了使用荧光染色观察细胞细胞器，以分析参与各种细胞事件的 CCN 家族蛋白的功能。
