Key Laboratory of Fermentation Engineering (Ministry of Education), Bioengineering and Food College, Hubei University of Technology, Wuhan, Hubei, China; National 111 Center for Cellular Regulation and Molecular Pharmaceutics, Wuhan, Hubei, China.
Key Laboratory of Fermentation Engineering (Ministry of Education), Bioengineering and Food College, Hubei University of Technology, Wuhan, Hubei, China; National 111 Center for Cellular Regulation and Molecular Pharmaceutics, Wuhan, Hubei, China.
Food Chem. 2023 Mar 30;405(Pt A):134817. doi: 10.1016/j.foodchem.2022.134817. Epub 2022 Nov 4.
In order to extract sulforaphane (SFN) from broccoli via green and efficient ways, a novel method based on salting-out assisted deep eutectic solvent (DES) has been developed. Compared to known organic solvent- (such as dichloromethane, ethyl acetate, n-hexane, etc.) based liquid-liquid extraction, this new NCl-based DES method exhibited excellent extraction efficiency for SFN, including a significant improvement due to the salting-out effect of KHPO. Under optimal conditions, 97.77 % of SFN was extracted by NCl-EG DES and more than 82.5 % of SFN was recovered by activated carbon from DES. In addition, further studies with Kamlet-Taft parameters and density functional theory showed that the H-bond accepting capacity of hydrophobic DES, the existing vdW interaction, and the electrostatic interaction between NCl-EG DES all contributed to efficient extraction of SFN. This is the first time that the underlying mechanism for SFN extraction by DES was revealed.
为了通过绿色高效的方法从西兰花中提取萝卜硫素（SFN），开发了一种基于加盐辅助深共晶溶剂（DES）的新方法。与已知的有机溶剂（如二氯甲烷、乙酸乙酯、正己烷等）为基础的液-液萃取相比，这种新的基于 NCl 的 DES 方法对 SFN 具有优异的萃取效率，由于 KHPO 的盐析效应，萃取效率显著提高。在最佳条件下，NCl-EG DES 可提取 97.77%的 SFN，而活性炭可从 DES 中回收超过 82.5%的 SFN。此外，通过 Kamlet-Taft 参数和密度泛函理论进一步研究表明，疏水性 DES 的氢键接受能力、存在的 vdW 相互作用以及 NCl-EG DES 之间的静电相互作用都有助于 SFN 的有效提取。这是首次揭示 DES 提取 SFN 的潜在机制。
