Enhanced hydrogen production from sludge anaerobic fermentation by combined freezing and calcium hypochlorite pretreatment.

A novel and high-efficiency sludge pretreatment method by combination of freezing and calcium hypochlorite (CH) for promoting the anaerobic fermentation performance was reported in this work. Experimental results indicated that a maximum biohydrogen production of 18.18 ± 0.43 mL/g volatile suspended solids (VSS) was realized by freezing (-5 °C) combined with CH (0.12 g/g VSS) pretreatment, which was 1.19, 4.05 and 11.36 times to that from the sole CH, sole freezing and control fermenters, respectively. Mechanism study showed that freezing + CH pretreatment efficiently disintegrated sludge flocs, producing abundant substrates for anaerobic fermentation. Model substances degradation experiment showed that the biochemical processes were all suppressed by freezing + CH method, but the suppressive degrees for hydrogen-consuming processes were greater than hydrogen-producing processes. 16S rRNA analysis revealed that the microbial community in freezing + CH treated reactor was more beneficial to hydrogen generation than that in the control, because the abundance of functional microbes was enriched from 6.81 % to 34.95 % by the co-treatment. Furthermore, sludge dewatering performance, including settleability, dewaterability and filterability, was enhanced by freezing + CH pretreatment.