Department of Physical Therapy, School of Health Sciences, Japan University of Health Sciences, Satte, Saitama 340-0145, Japan; Graduate School of Health Sciences, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Graduate School of Health Sciences, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Exp Gerontol. 2023 Jan;171:112024. doi: 10.1016/j.exger.2022.112024. Epub 2022 Nov 11.
The decline in spatial working memory is one of the earliest signs of normal brain aging.
We developed a novel physical exercise method, termed the "shaking exercise," to slow down this process.
The experimental protocol included administering the shaking exercise for 8-32 weeks in male senescence-accelerated mouse prone 10 (SAMP-10). They were subjected to the T-maze test, followed by immunohistochemical analysis, to assess the influence of the shaking exercise on the M1 muscarinic acetylcholine receptor (CHRM1) and α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) of the dorsal hippocampus and medial prefrontal cortex (dHC-mPFC).
The T-maze test demonstrated that the shaking group had less hesitation in the face of selecting direction at week 24. In the immunohistochemical analysis, more CHRM1s were in the CA3 subregion and more AMPARs were in the subiculum. CHRM1s and AMPARs were maintained in the CA1 region and the mPFC. The CHRM1s seem to have a positive effect on the AMPAR in the dentate gyrus (DG) region and the CA3 region. In the CA1 region, CHRM1s were negatively correlated with AMPARs. In addition, high-density neurons were expressed in the shaking group in the upstream DG, the middle part and the distal part of CA3, the distal part of CA1, and the mPFC.
Our results raise the possibility that maintenance of the spatial working memory effect observed with the shaking exercise is driven in part by the uneven affection of CHRM1s and AMPARs in the dHC-mPFC circuit system and significantly maintains the neuronal expression in the dHC-mPFC.
空间工作记忆的下降是正常大脑衰老的最早迹象之一。
我们开发了一种新的体育锻炼方法，称为“摇动锻炼”，以减缓这一过程。
实验方案包括对雄性衰老加速小鼠易感 10 号（SAMP-10）进行 8-32 周的摇动锻炼。他们接受了 T 迷宫测试，随后进行了免疫组织化学分析，以评估摇动锻炼对背海马和内侧前额叶皮质（dHC-mPFC）中 M1 毒蕈碱乙酰胆碱受体（CHRM1）和 α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体（AMPAR）的影响。
T 迷宫测试表明，摇动组在第 24 周面对选择方向时犹豫较少。在免疫组织化学分析中，CA3 亚区有更多的 CHRM1，而 subiculum 中有更多的 AMPAR。CHRM1 和 AMPAR 维持在 CA1 区域和 mPFC 中。CHRM1 似乎对齿状回（DG）区域和 CA3 区域的 AMPAR 有积极影响。在 CA1 区域，CHRM1 与 AMPAR 呈负相关。此外，在摇动组中，上游 DG、CA3 的中部和远端、CA1 的远端以及 mPFC 中表达了高密度神经元。
我们的结果提出了一种可能性，即摇动锻炼观察到的空间工作记忆效应的维持部分是由 dHC-mPFC 电路系统中 CHRM1 和 AMPAR 的不均匀影响驱动的，并显著维持了 dHC-mPFC 中的神经元表达。
