College of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou, 730000, China.
Department of Pathology, the 940th Hospital of the Joint Logistic Support of the People's Liberation Army, Lanzhou, 730050, China.
World J Surg Oncol. 2022 Nov 14;20(1):362. doi: 10.1186/s12957-022-02830-9.
Gastric cancer has a high incidence and mortality rate. Angiogenesis is necessary for tumor infiltration and metastasis and affects patient prognosis. YKL-39 has monocyte chemotactic activity and pro-angiogenic activity in some tumors. In this study, we investigated the relationship between YKL-39 and tumor-associated macrophages and microangiogenesis in gastric cancer to determine its potential as a prognostic biomarker.
A total of 119 patients with gastric cancer who had undergone gastrectomy at the 940th Hospital of the Joint Security Force between 2014 and 2018 were included in this study. We assayed the protein expression of YKL-39, CD68, and CD34 by immunohistochemistry in tissues of 119 patients with gastric cancer, as well as the intracellular expression of YKL-39 and CD68 by immunofluorescence. Data were analyzed with SPSS Statistics 25.0 to explore the impact of expression of YKL-39, CD68, and CD34 in gastric cancer patients and the relationship among them.
Our results show that YKL-39 was expressed in both the nucleus and cytoplasm of gastric cancer cells and tumor mesenchyme. YKL-39 protein expression was associated with the depth of tumor infiltration, lymph node metastasis, and TNM stage; CD68 protein expression was associated with lymph node metastasis and TNM stage; CD34 protein expression was not associated with clinicopathological characteristics. Expression of YKL-39 was positively correlated with CD68 and CD34 (p < 0.001), and high expression of YKL-39 was associated with poor prognosis (p < 0.05).
In gastric cancer, YKL-39 expression is positively correlated with the degree of tumor-associated macrophage infiltration and angiogenesis, and is a potential prognostic marker for gastric cancer.
胃癌具有较高的发病率和死亡率。血管生成对于肿瘤浸润和转移是必需的，并影响患者的预后。YKL-39 在一些肿瘤中具有单核细胞趋化活性和促血管生成活性。在这项研究中，我们研究了 YKL-39 与胃癌中肿瘤相关巨噬细胞和微血管生成的关系，以确定其作为预后生物标志物的潜力。
本研究纳入了 2014 年至 2018 年期间在 940 联合安全部队医院接受胃切除术的 119 例胃癌患者。我们通过免疫组织化学法检测了 119 例胃癌患者组织中 YKL-39、CD68 和 CD34 的蛋白表达，并通过免疫荧光法检测了 YKL-39 和 CD68 的细胞内表达。使用 SPSS Statistics 25.0 进行数据分析，以探讨 YKL-39、CD68 和 CD34 在胃癌患者中的表达及其之间的关系。
我们的结果表明，YKL-39 在胃癌细胞和肿瘤间质的核和细胞质中均有表达。YKL-39 蛋白表达与肿瘤浸润深度、淋巴结转移和 TNM 分期有关；CD68 蛋白表达与淋巴结转移和 TNM 分期有关；CD34 蛋白表达与临床病理特征无关。YKL-39 的表达与 CD68 和 CD34 呈正相关（p<0.001），且 YKL-39 高表达与预后不良相关（p<0.05）。
在胃癌中，YKL-39 的表达与肿瘤相关巨噬细胞浸润和血管生成程度呈正相关，是胃癌的潜在预后标志物。
