Department of Food Science and Biotechnology, Sungkyunkwan University (SKKU), 2066 Seobu-ro, Jangan-gu, Suwon 16419, Republic of Korea.
BioFoundry Research Center, Institute of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), 2066 Seobu-ro, Jangan-gu, Suwon 16419, Republic of Korea.
J Agric Food Chem. 2022 Nov 23;70(46):14755-14760. doi: 10.1021/acs.jafc.2c05562. Epub 2022 Nov 14.
is widely used for a large-scale industrial producer of feed additive amino acids, such as l-lysine. Moreover, has been engineered for producing various non-native chemicals, including terpenes. For the first time, was engineered for co-production of l-lysine and heterologous squalene. To control metabolic fluxes for either the l-lysine biosynthesis pathway or the squalene biosynthesis pathway, pyruvate, an intermediate in the central metabolism, a node was regulated by a clustered regularly interspaced short palindromic repeat (CRISPR) interference system. Repressing encoding for pyruvate carboxylase in the l-lysine producer (DM1919) and its derivatives resulted in 99.24 ± 7.63 mg/L total squalene and 6.25 ± 0.20 g/L extracellular lysine at 120 h. Furthermore, various oil overlays were tested for efficient co-productions. extraction with corn oil (10%, v/v) exhibited a separation of 99.75% (w/v) of total squalene (intra- and extracellular squalene), while l-lysine can be secreted in the medium. This co-production strategy will help a potential bioprocess of amino acid production with various terpenes.
被广泛应用于大规模工业生产饲料添加剂氨基酸，如赖氨酸。此外，它还被用于生产各种非天然化学品，包括萜类化合物。这是第一次，被用于共生产赖氨酸和异源角鲨烯。为了控制中央代谢途径中的丙酮酸中间体的赖氨酸生物合成途径或角鲨烯生物合成途径的代谢通量，一个节点被一个成簇规律间隔短回文重复（CRISPR）干扰系统调控。在赖氨酸生产菌（DM1919）及其衍生物中抑制编码丙酮酸羧化酶的基因，可导致在 120 小时内总角鲨烯达到 99.24 ± 7.63 mg/L，胞外赖氨酸达到 6.25 ± 0.20 g/L。此外，还测试了各种油覆盖物以实现高效共生产。用玉米油（10%，v/v）萃取可实现总角鲨烯（胞内和胞外角鲨烯）的 99.75%（w/v）分离，而赖氨酸可以分泌到培养基中。这种共生产策略将有助于实现氨基酸生产与各种萜类化合物的潜在生物工艺。
