Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health. Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture. Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction. The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film. Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties. Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film. Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.