Department of Medical Laboratory Techniques, Vocational School of Health Services, Tokat Gaziosmanpasa University, 60250 Tokat, Turkey.
Department of Bioengineering, Faculty of Engineering and Architecture, Tokat Gaziosmanpasa University, 60250 Tokat, Turkey.
Bioorg Chem. 2023 Jan;130:106230. doi: 10.1016/j.bioorg.2022.106230. Epub 2022 Nov 8.
Colorectal cancer is a type of cancer encountered worldwide and ranks third among all cancer types in terms of incidence. Polyphenols have been shown to have a wide range of biological functions, including a significant impact on cancer start, development, and promotion through regulating many signaling pathways. The aim of this study was to investigate the anticancer effects of isoeugenol based compounds 1, 2 on HT29 colorectal cancer cell line in vitro. MTT test and scratch assay were carried out to determine the effect of these compounds on HT29 cell proliferation and migration respectively. In addition, mRNA expression levels of apoptosis and metastasis-related genes (p53, Bcl2, Bax, Caspase 3, Caspase7, Caspase8, Caspase9, HIF1-α, VEGF, MMP-2, MMP-9) were examined by quantitative real-time PCR. The results indicated that 1 and 2 inhibited HT29 cell proliferation and induced apoptosis by increasing the Bax/Bcl2 ratio and Caspase-9 and Caspase-3 mRNA expression. In conclusion, the results of this study showed that the treatment of these compounds significantly suppressed the mRNA expressions of metastasis-related genes such as Matrix Metalloproteinase-2, Matrix Metalloproteinase-9, Vascular Endothelial Growth Factor and Hypoxia‑Inducible Factor 1α.
结直肠癌是一种在全球范围内都有发现的癌症，其发病率在所有癌症类型中排名第三。多酚具有广泛的生物学功能，包括通过调节许多信号通路对癌症的起始、发展和促进产生重大影响。本研究旨在探讨异丁香酚基化合物 1、2 对 HT29 结直肠癌细胞系的体外抗癌作用。通过 MTT 试验和划痕试验分别测定这些化合物对 HT29 细胞增殖和迁移的影响。此外，通过定量实时 PCR 检测凋亡和转移相关基因（p53、Bcl2、Bax、Caspase 3、Caspase7、Caspase8、Caspase9、HIF1-α、VEGF、MMP-2、MMP-9）的 mRNA 表达水平。结果表明，1 和 2 通过增加 Bax/Bcl2 比值和 Caspase-9 和 Caspase-3 mRNA 表达抑制 HT29 细胞增殖并诱导细胞凋亡。综上所述，本研究结果表明，这些化合物的治疗显著抑制了基质金属蛋白酶-2、基质金属蛋白酶-9、血管内皮生长因子和缺氧诱导因子 1α 等转移相关基因的 mRNA 表达。
