Department of Hospital Infection Management, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China.
Department of Critical Care Medicine, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China; Department of Nursing, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China.
Am J Infect Control. 2023 Mar;51(3):276-281. doi: 10.1016/j.ajic.2022.10.017. Epub 2022 Nov 12.
To evaluate potential viral contamination on the surfaces of personal protective equipment (PPE) in COVID-19 wards.
Face shields, gloves, the chest area of PPE and shoe soles were sampled at different time points. The samples were tested for the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by PCR, and the cycle threshold (CT) values were recorded.
The positive rate was 74.7% (239/320) for all PPE specimens. The CT values of the samples were ranked in the following order: face shields > chests > gloves > shoe soles (37.08±1.38, 35.48±2.02, 34.17±1.91 and 33.52±3.16, respectively; P for trend < .001). After disinfection, the CT values of shoe soles decreased compared with before disinfection (32.78±3.47 vs. 34.3±2.61, P = .037), whereas no significant effect of disinfection on the CT values of face shields, chests and gloves was observed. After disinfection, the CT values of specimens collected from shoe soles gradually increased; before disinfection, the CT values of shoe sole specimens were all less than 35.
SARS-CoV-2 can attach to the surfaces of the PPE of healthcare professionals in COVID-19 wards, especially the shoe soles and undisinfected gloves. Shoe soles had the highest SARS-CoV-2 loads among all tested PPE items.
评估 COVID-19 病房中个人防护装备（PPE）表面的潜在病毒污染情况。
在不同时间点采集面罩、手套、PPE 胸部区域和鞋底的样本。通过 PCR 检测样本中是否存在严重急性呼吸综合征冠状病毒 2（SARS-CoV-2），并记录循环阈值（CT）值。
所有 PPE 标本的阳性率为 74.7%（239/320）。样本的 CT 值按以下顺序排列：面罩>胸部>手套>鞋底（37.08±1.38、35.48±2.02、34.17±1.91 和 33.52±3.16；趋势 P<0.001）。消毒后，鞋底的 CT 值较消毒前降低（32.78±3.47 比 34.3±2.61，P=0.037），而消毒对面罩、胸部和手套的 CT 值没有显著影响。消毒后，鞋底标本的 CT 值逐渐升高；消毒前，鞋底标本的 CT 值均小于 35。
SARS-CoV-2 可附着在 COVID-19 病房中医护人员 PPE 的表面，尤其是鞋底和未消毒的手套。所有检测的 PPE 物品中，鞋底的 SARS-CoV-2 载量最高。
