Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, PO Box 273, Banjul, The Gambia.
National Reference Laboratory, Nigeria Centre for Disease Control and Preventiuon, Abuja, Nigeria.
Sci Rep. 2022 Nov 14;12(1):19523. doi: 10.1038/s41598-022-22777-x.
The clinical relevance of pulmonary non-tuberculous mycobacteria (PNTM) in The Gambia is unknown. The aim of this study was to estimate the prevalence of non-tuberculous mycobacteria (NTM) in colonisation, and the burden of clinically relevant pulmonary NTM (PNTM) disease in The Gambia. This was a cross-sectional study of the prevalence of NTM in participants aged ≥ 15 years, in a nationwide tuberculosis (TB) prevalence survey between December 2011 and January 2013. We enrolled 903 participants with suspected NTM and NTM cultures were confirmed by 16S rRNA gene sequencing analyses. We applied the American Thoracic Society/Infectious Disease Society of America (ATS/IDSA) diagnostic criteria to determine clinical relevance of NTM. A total of 575 participants had acid-fast bacilli (AFB) positive Mycobacterial Growth Indicator Tube (MGIT) cultures and 229 (39.8%) were NTM. M. avium complex was by far the most isolated NTM (71.0%), followed by M. fortuitum (9.5%) and M. nonchromogenicum (2.9%). Older participants (> 24 years old) were four times more likely to have NTM in their sputa. Only 20.5% (9/44) NTM cases met the ATS/IDSA criteria for NTM disease. This study provides important data on the prevalence of NTM in pulmonary samples of suspected TB cases with AFB positive cultures from a nationally representative population in The Gambia. Enhanced PNTM surveillance is recommended to better understand the contribution of NTM to pulmonary disease.
冈比亚尚不清楚非结核分枝杆菌（NTM）在肺部的临床相关性。本研究旨在评估分枝杆菌在人群中的定植率，以及冈比亚有临床意义的肺部非结核分枝杆菌（PNTM）疾病的负担。这是一项横断面研究，在 2011 年 12 月至 2013 年 1 月期间进行的全国结核病（TB）患病率调查中，对年龄≥15 岁的参与者中 NTM 的患病率进行了研究。我们招募了 903 名疑似 NTM 患者，通过 16S rRNA 基因测序分析证实了 NTM 培养物。我们应用美国胸科学会/传染病学会（ATS/IDSA）诊断标准来确定 NTM 的临床相关性。共有 575 名参与者的抗酸杆菌（AFB）阳性分枝杆菌生长指示管（MGIT）培养物为阳性，其中 229 名（39.8%）为 NTM。迄今为止，MAIC 是最常分离到的 NTM（71.0%），其次是 FM（9.5%）和 MNC（2.9%）。年龄较大的参与者（>24 岁）更有可能在其痰液中存在 NTM。只有 20.5%（9/44）的 NTM 病例符合 ATS/IDSA 对 NTM 疾病的标准。本研究提供了重要数据，表明在冈比亚具有 AFB 阳性培养的疑似结核病病例的肺部样本中，NTM 的患病率很高。建议加强对 PNTM 的监测，以更好地了解 NTM 对肺部疾病的贡献。
