与苦瓜抵抗蔓枯病菌（Stagonosporopsis citrulli）相关的 QTL 分析。
QTL associated with resistance to Stagonosporopsis citrulli in Citrullus amarus.
机构信息
Institute of Plant Breeding, Genetics, and Genomics, University of Georgia, Athens, GA, USA.
出版信息
Sci Rep. 2022 Nov 15;12(1):19628. doi: 10.1038/s41598-022-23704-w.
Gummy stem blight (GSB) is a fungal disease affecting cucurbit crops, including watermelon (Citrullus lanatus), leading to significant yield losses. The disease is caused by three Stagonosporopsis species, of which Stagonosporopsis citrulli is the most common in the southeastern United States. Currently no gummy stem blight-resistant watermelon cultivars are available to growers. In this study, QTL-seq in an interspecific population developed from Sugar Baby × PI 189225 (Citrullus amarus) identified QTL on chromosomes 2, 5, 9 and 11. A novel QTL on chromosome 5 (Qgsb5.2) associated with resistance to S. citrulli (PVE = 13.3%) was confirmed by genetic mapping. KASP marker assays were developed for selection of Qgsb5.2 to allow breeders to track the allele contributing resistance to GSB, reducing the need for laborious phenotyping. Pyramiding different GSB resistance QTL could be a useful strategy to develop GSB resistant watermelon cultivars.
胶质茎枯病（GSB）是一种影响葫芦科作物的真菌病，包括西瓜（Citrullus lanatus），导致严重的产量损失。该病由三种 Stagonosporopsis 种引起，其中在美国东南部最常见的是 Stagonosporopsis citrulli。目前，种植者无法获得抗胶质茎枯病的西瓜品种。在这项研究中，来自 Sugar Baby × PI 189225（Citrullus amarus）的种间群体的 QTL-seq 鉴定出了 2、5、9 和 11 号染色体上的 QTL。通过遗传作图证实了与 S. citrulli（PVE = 13.3%）抗性相关的 5 号染色体上的一个新 QTL（Qgsb5.2）。为了选择 Qgsb5.2，开发了 KASP 标记分析，使育种者能够跟踪对 GSB 有抗性的等位基因，减少了费力的表型选择。聚合不同的 GSB 抗性 QTL 可能是开发抗 GSB 西瓜品种的一种有用策略。
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