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利用 QTL-seq 鉴定与南瓜抗蔓枯病相关的基因座。

QTL-seq for identification of loci associated with resistance to Phytophthora crown rot in squash.

机构信息

Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.

出版信息

Sci Rep. 2020 Mar 24;10(1):5326. doi: 10.1038/s41598-020-62228-z.

DOI:10.1038/s41598-020-62228-z
PMID:32210312
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7093484/
Abstract

Phytophthora capsici Leonian, the causal agent of foliar blight, root rot, fruit rot and crown rot syndromes in squash (Cucurbita moschata), is a devastating pathogen worldwide. Resistance to Phytophthora crown rot in University of Florida breeding line #394-1-27-12 (C. moschata) is conferred by three independent dominant genes (R1R2R3). Availability of DNA markers linked to R1R2R3 genes would allow efficient breeding for Phytophthora crown rot resistance through marker-assisted selection (MAS). The goal of the current study was to identify quantitative trait loci (QTLs) associated with resistance to Phytophthora crown rot in an F population (n = 168) derived from a cross between #394-1-27-12 (R) and Butter Bush (S) using QTL-seq bulk segregant analysis. Whole-genome resequencing of the resistant (n = 20) and susceptible (n = 20) bulk segregants revealed ~900,000 single nucleotide polymorphisms distributed across C. moschata genome. Three QTLs significantly (P < 0.05) associated with resistance to Phytophthora crown rot were detected on chromosome 4 (QtlPC-C04), 11 (QtlPC-C11) and 14 (QtlPC-C14). Several markers linked to these QTLs are potential targets for MAS against Phytophthora crown rot in C. moschata. The present study reports the first QTLs associated with Phytophthora crown rot resistance in C. moschata.

摘要

辣椒疫霉（Phytophthora capsici Leonian）是一种毁灭性的病原体，可引起南瓜（Cucurbita moschata）的叶斑病、根腐病、果腐病和冠腐病。佛罗里达大学的 394-1-27-12 品系（C. moschata）对辣椒疫霉冠腐病具有抗性，这种抗性由三个独立的显性基因（R1R2R3）赋予。与 R1R2R3 基因连锁的 DNA 标记的可用性将允许通过标记辅助选择（MAS）有效地培育对辣椒疫霉冠腐病的抗性。本研究的目的是使用 QTL-seq bulk segregant 分析，从 394-1-27-12（R）和 Butter Bush（S）杂交后代的 F 群体（n=168）中鉴定与辣椒疫霉冠腐病抗性相关的数量性状位点（QTL）。对抗性（n=20）和易感（n=20） bulk segregant 进行全基因组重测序，揭示了约 90 万个分布在 C. moschata 基因组中的单核苷酸多态性。在第 4 号染色体（QtlPC-C04）、第 11 号染色体（QtlPC-C11）和第 14 号染色体（QtlPC-C14）上检测到与辣椒疫霉冠腐病抗性显著相关的三个 QTL（P<0.05）。与这些 QTL 相关的几个标记是 C. moschata 中针对辣椒疫霉冠腐病 MAS 的潜在目标。本研究报道了与 C. moschata 中辣椒疫霉冠腐病抗性相关的第一个 QTL。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/f3c58b330c77/41598_2020_62228_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/104a98bac5bf/41598_2020_62228_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/4f0381f93da1/41598_2020_62228_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/6a7ec9c8c647/41598_2020_62228_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/f3c58b330c77/41598_2020_62228_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/104a98bac5bf/41598_2020_62228_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/4f0381f93da1/41598_2020_62228_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/6a7ec9c8c647/41598_2020_62228_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b4/7093484/f3c58b330c77/41598_2020_62228_Fig4_HTML.jpg

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