Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Department of Clinical Chemistry, Iranian Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, P.O. Box: 14665-1157, Tehran, Iran.
J Thromb Thrombolysis. 2023 Jan;55(1):60-66. doi: 10.1007/s11239-022-02725-2. Epub 2022 Nov 15.
Platelet storage lesions may occur in Platelet concentrates (PCs) storage time, reducing PCs' quality. Mitochondrial damage causes mitochondrial DNA (mtDNA) to be released into the extracellular space. In this study, we evaluated the effect of L-carnitine (LC) as an antioxidant on free mtDNA DAMPs release in PCs during storage. Ten PCs prepared by the PRP method were studied. The copy numbers of free mtDNA, total reactive oxygen species (ROS), lactate dehydrogenase (LDH) enzyme activity, pH, and platelet counts were measured on days 0, 3, 5, and 7 of PCs storage in LC-treated and untreated platelets. LDH activity was significantly lower than the control group during 7 days of PCs storage (p = 0.041). Also, ROS production decreased in LC-treated PCs compared to the control group during storage (p = 0.026), and the difference mean of ROS between the two groups was significant on day 3, 5, and 7 (P = 0.02, P = 0.0001, P = 0.031). Moreover, LC decreased the copy numbers of free mtDNA during 7 days of storage (p = 0.021), and the difference mean of the copy numbers of free mtDNA in LC-treated PCs compared to the control group was significant on day 5 and 7 (P = 0.041، P = 0.022). It seems that LC can maintain the metabolism and antioxidant capacity of PCs and thus can reduce mitochondrial damage and mtDNA release; consequently, it can decrease DAMPs in PCs. Therefore, it may be possible to use this substance as a platelet additive solution in the future.
血小板储存损伤可能发生在血小板浓缩物 (PCs) 的储存时间内，从而降低 PCs 的质量。线粒体损伤导致线粒体 DNA (mtDNA) 释放到细胞外空间。在这项研究中，我们评估了左旋肉碱 (LC) 作为抗氧化剂对储存过程中 PCs 中游离 mtDNA DAMPs 释放的影响。研究了通过 PRP 方法制备的 10 份 PCs。在 LC 处理和未处理的血小板中，分别在 PCs 储存的第 0、3、5 和 7 天测量游离 mtDNA 的拷贝数、总活性氧 (ROS)、乳酸脱氢酶 (LDH) 酶活性、pH 值和血小板计数。在 PCs 储存的 7 天内，LDH 活性明显低于对照组（p = 0.041）。此外，与对照组相比，LC 处理的 PCs 在储存过程中 ROS 生成减少（p = 0.026），且两组之间 ROS 的差异均值在第 3、5 和 7 天具有统计学意义（P = 0.02、P = 0.0001、P = 0.031）。此外，LC 在 7 天的储存期间降低了游离 mtDNA 的拷贝数（p = 0.021），并且 LC 处理的 PCs 与对照组之间游离 mtDNA 拷贝数的差异均值在第 5 和 7 天具有统计学意义（P = 0.041，P = 0.022）。似乎 LC 可以维持 PCs 的代谢和抗氧化能力，从而减少线粒体损伤和 mtDNA 释放；因此，可以减少 PCs 中的 DAMPs。因此，将来可能可以将这种物质用作血小板添加剂溶液。
