Sp4 调节基因转录和表达。

Sp4 Regulates Gene Transcription and Expression.

机构信息

Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Institute of Aging, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Zhejiang Provincial Clinical Research Center for Mental Disorders, School of Mental Health and Kangning Hospital, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

出版信息

DNA Cell Biol. 2022 Dec;41(12):1053-1062. doi: 10.1089/dna.2022.0243. Epub 2022 Nov 16.

DOI:10.1089/dna.2022.0243

PMID:36383136

Abstract

Pituitary tumor-transforming gene 1 protein (PTTG)-interacting protein, also known as PTTG-binding factor (PBF), is encoded by a proto-oncogene . PBF has been identified through its interaction with PTTG. Similar to PTTG, PBF has been implicated in the etiology of several tumors, including pituitary, thyroid, and breast cancer. PBF can induce the translocation of PTTG into the nucleus, and then lead to tumorigenesis. Studies have shown that PBF plays a vital and complex role in increasing tumor development. However, the transcriptional regulation of gene remains undefined. In this study, we have cloned a 467-bp fragment of the 5' flanking region of the human gene and identified the region (-212 to +7 bp) necessary for gene promoter activity by luciferase assay. Electrophoretic mobility shift assay revealed gene promoter containing Sp4 response elements. Overexpression of Sp4 increased gene transcription and expression in HeLa cells. Our study demonstrates that Sp4 regulates gene transcription and expression.

摘要

垂体肿瘤转化基因 1 蛋白（PTTG）相互作用蛋白，也称为 PTTG 结合因子（PBF），由原癌基因编码。PBF 通过与 PTTG 的相互作用而被鉴定。与 PTTG 相似，PBF 与几种肿瘤的病因有关，包括垂体瘤、甲状腺癌和乳腺癌。PBF 可以诱导 PTTG 向核内易位，从而导致肿瘤发生。研究表明，PBF 在增加肿瘤发展方面起着至关重要和复杂的作用。然而，该基因的转录调控仍未确定。在这项研究中，我们克隆了人基因 5'侧翼区的 467bp 片段，并通过荧光素酶测定鉴定了启动子活性所必需的区域（-212 至+7bp）。电泳迁移率变动分析显示，基因启动子含有 Sp4 反应元件。Sp4 的过表达增加了 HeLa 细胞中基因的转录和表达。我们的研究表明，Sp4 调节基因的转录和表达。