Jirsova K, Levova K, Kalousova M, Fales I, Frankova V, Vesela V, Zima T, Utheim T P, Bednar J
Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.
Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.
Folia Biol (Praha). 2022;68(2):45-49. doi: 10.14712/fb2022068020045.
Autologous serum eye drops (ASEDs) are used as a treatment for severe dry eye disease. The concentration and stability of various growth factors in ASEDs is determinative for their efficiency. We therefore assessed the concentrations of transforming growth factor beta 1 (TGF-β1), epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) in ASEDs following storage at 4-8, -20, -80 and -156 °C. Twenty % and 100% sera from eight healthy volunteers were analysed by the sandwich enzyme immunoassay at different time intervals up to seven months. The mean levels of TGF-β1 and EGF in undiluted and 20% serum did not differ significantly from the baseline levels in fresh serum for any storage conditions after 7 days at 4-8 °C, as well as after 4- and 7-month preservation at sub-zero temperatures. In 20% serum, no IGF-1 concentration decrease was found following 7 days of preservation at 4-8 °C. However, a decrease to 78 % and 81 % (P < 0.01) of baseline values was found in 20% serum after 4-month storage at -20 °C and 7-month storage at -156 °C, respectively. A more pronounced decrease in IGF-1 was observed in undiluted serum. All assessed growth factors present in 20% frozen serum remained stable for up to 7 months. The highest stability was achieved at -80 °C. At -20 and -156 °C, some decrease in IGF-1 occurred. Our results indicate that 20% ASEDs can be stored frozen up to 7 months under proper conditions.
自体血清滴眼液（ASEDs）被用作严重干眼症的一种治疗方法。ASEDs中各种生长因子的浓度和稳定性决定了它们的疗效。因此，我们评估了在4-8℃、-20℃、-80℃和-156℃储存后ASEDs中转化生长因子β1（TGF-β1）、表皮生长因子（EGF）和胰岛素样生长因子1（IGF-1）的浓度。对来自8名健康志愿者的20%和100%血清在不同时间间隔直至7个月进行夹心酶免疫测定分析。在4-8℃保存7天后以及在零下温度保存4个月和7个月后，对于任何储存条件，未稀释和20%血清中TGF-β1和EGF的平均水平与新鲜血清中的基线水平相比均无显著差异。在20%血清中，4-8℃保存7天后未发现IGF-1浓度降低。然而，在20%血清中，分别在-20℃储存4个月和-156℃储存7个月后，IGF-1浓度降至基线值的78%和81%（P<0.01）。在未稀释血清中观察到IGF-1有更明显的降低。20%冷冻血清中存在的所有评估生长因子在长达7个月内保持稳定。在-80℃时稳定性最高。在-20℃和-156℃时，IGF-1出现了一些降低。我们的结果表明，20%的ASEDs在适当条件下可冷冻保存长达7个月。
