Wang Yiping, Kumar Kishore Ramesh, Liehr Thomas
Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Am Klinikum 1, 07747, Jena, Germany.
Jinze Group Beijing Jinze Medical Laboratory, Building A, National Engineering Center for Protein Drugs, Building 1, 33 Science Park Road, Huilongguan Town, Changping District, Beijing, 1F, China.
Mol Cytogenet. 2022 Nov 16;15(1):50. doi: 10.1186/s13039-022-00628-8.
Molecular combing technology (MCT) is an effective means for stretching DNA molecules and making them thus accessible for in situ studies. MCT uses the force exerted in the process of liquid flow via surface tension to stretch DNA molecules and spread them on solid surfaces, i.e. glass cover slips. Many DNA molecules can be stretched at the same time in parallel and neatly arranged side-by-side, making the approach convenient for statistical analysis. Accordingly, DNA replication and transcription can be studied at the single molecule level. In this paper, the principle, experimental methods, important applications, advantages and shortcuts of MCT in medical field are presented and discussed.
分子梳技术（MCT）是一种拉伸DNA分子并使其可用于原位研究的有效手段。MCT利用液体流动过程中通过表面张力施加的力来拉伸DNA分子，并将其铺展在固体表面，即玻璃盖玻片上。许多DNA分子可以同时被平行拉伸并并排整齐排列，这使得该方法便于进行统计分析。因此，可以在单分子水平上研究DNA复制和转录。本文介绍并讨论了MCT在医学领域的原理、实验方法、重要应用、优点和不足。
