Chen Bing-Bing, Sun Zhi-Gao, Hu Xing-Yun, Wu Hui-Hui, Wang Xiao-Ying, Li Mao, Li Yan-Zhe
Ministry of Education Key Laboratory of Humid Subtropical Eco-geographical Process, Fujian Normal University, Fuzhou 350007, China.
Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, China.
Ying Yong Sheng Tai Xue Bao. 2022 Oct;33(11):3007-3015. doi: 10.13287/j.1001-9332.202211.016.
To explore the differences in structure and diversity of -type denitrifying microbial community in marsh soils at different invasion stages of , the mudflat (MF, before invasion) and the marsh after seaward invasion for 1-2 years (SAN) and 6-7 years (SA) in Shanyutan of the Minjiang River estuary were investigated by high-through put sequencing method. Results showed that the seaward invasion of reduced the richness and diversity of -type denitrifying microbial community in marsh soils. The -type denitrifying microbial community in soils at different invasion stages included Proteobacteria and Actinobacteria, with Proteobacteria as the dominant one. The seaward invasion of greatly altered the composition of -type denitrifying microbial community in marsh soils. The highest relative abundance of genus in soils from different invasion stages were , and , respectively. The seaward invasion of increased the spatial heterogeneity of -type denitrifying microbial community composition in marsh soils. In SAN plot, the enhancement of spatial heterogeneity was primarily due to higher environmental disturbances in plots and the increased spatial heterogeneity of environmental variables caused by the seaward invasion of . The seaward invasion of altered the physico-chemical properties (, grain composition, pH and moisture) and N nutrient conditions (total N, NH-N and NO-N) in marsh soils, which greatly altered the structure and diversity of -type denitrifying microbial community. Our findings reveal the microbial mechanism of denitrification process in marsh soils during the seaward invasion of .
为探究在不同入侵阶段的滨海湿地土壤中反硝化微生物群落的结构和多样性差异，采用高通量测序方法对闽江河口鳝鱼滩的泥滩（MF，入侵前）以及向海入侵1 - 2年（SAN）和6 - 7年（SA）后的滨海湿地进行了调查。结果表明，的向海入侵降低了滨海湿地土壤中反硝化微生物群落的丰富度和多样性。不同入侵阶段土壤中的反硝化微生物群落包括变形菌门和放线菌门，其中变形菌门为优势菌门。的向海入侵极大地改变了滨海湿地土壤中反硝化微生物群落的组成。不同入侵阶段土壤中属的最高相对丰度分别为、和。的向海入侵增加了滨海湿地土壤中反硝化微生物群落组成的空间异质性。在SAN样地中，空间异质性的增强主要是由于样地中较高的环境干扰以及的向海入侵导致环境变量空间异质性增加。的向海入侵改变了滨海湿地土壤的理化性质（、颗粒组成、pH值和湿度）和氮养分状况（总氮、铵态氮和硝态氮），这极大地改变了反硝化微生物群落的结构和多样性。我们的研究结果揭示了在向海入侵过程中滨海湿地土壤反硝化过程的微生物机制。
