Sharifnezhad Amir Hossein, Dashtian Kheibar, Zare-Dorabei Rouholah, Mahdavi Mohammad
Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran.
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Mikrochim Acta. 2022 Nov 17;189(12):448. doi: 10.1007/s00604-022-05554-5.
A photo-induced metal-organic framework-enzyme hybrid nanosystem was developed via a controllable physical embedding method that displays dual enzymatic and photo-non-enzymatic strategy which cause high stability and cascade catalytic performance to oxidation of o-phenylenediamine and generate a UV-Vis signal at 450 nm for the tracing and sensitive detection of putrescine (Put). Under optimal conditions, the present bioassay provides a wide detection range from 0.02 to 10 µM and 20-80 µM with a detection limit of 5.5 nM, which is more desirable than numerous previous reports. In addition, the established colorimetric photo-bioassay can selectively and accurately identify Put in the presence of other distributing species. The present work provides an elegant strategy to merged photo-nanozymes' and enzyme capabilities and also broadened the sensing strategies of photo-nanozymes with promising potential in the realm of cancer diagnosis and food quality monitoring as well as its potential in various bioassays and heterogeneous catalysis fields.
通过可控的物理嵌入方法构建了一种光诱导金属有机框架-酶杂化纳米系统，该系统展示了双重酶促和光非酶促策略，对邻苯二胺氧化具有高稳定性和级联催化性能，并在450nm处产生紫外可见信号，用于腐胺（Put）的追踪和灵敏检测。在最佳条件下，本生物测定法的检测范围为0.02至10µM和20至80µM，检测限为5.5 nM，比之前的众多报道更具优势。此外，所建立的比色光生物测定法能够在存在其他分布物种的情况下选择性且准确地识别Put。本工作提供了一种巧妙的策略，将光纳米酶和酶的能力相结合，同时拓宽了光纳米酶的传感策略，在癌症诊断、食品质量监测领域具有广阔的潜力，以及在各种生物测定和多相催化领域的潜力。
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