State Key Laboratory of Analytical Chemistry for Life Science; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Department of Chemistry, China Pharmaceutical University, Nanjing 211198, China.
Anal Chem. 2021 Jun 22;93(24):8544-8552. doi: 10.1021/acs.analchem.1c01261. Epub 2021 Jun 7.
Bacterial infection is one of the major causes of human death worldwide. To prevent bacterial infectious diseases from spreading, it is of critical importance to develop convenient, ultrasensitive, and cost-efficient methods for bacteria detection. Here, an electrochemical detector of a functional two-dimensional (2D) metal-organic framework (MOF) nanozyme was developed for the sensitive detection of pathogenic . A dual recognition strategy consisting of vancomycin and anti- antibody was proposed to specifically anchor . The 2D MOFs with excellent peroxidase-like activity can efficiently catalyze -phenylenediamine to 2,2-diaminoazobenzene, which is an ideal electrochemical signal readout for monitoring the bacteria concentration. Under optimal conditions, the present bioassay provides a wide detection range of 10-7.5 × 10 colony-forming units CFU/mL with a detection limit of 6 CFU/mL, which is better than most of the previous reports. In addition, the established electrochemical sensor can selectively and accurately identify in the presence of other bacteria. The present work provides a new pathway for sensitive and selective detection of and presents a promising potential in the realm of clinical diagnosis.
细菌感染是全球人类死亡的主要原因之一。为了防止细菌传染病的传播，开发方便、高灵敏度和低成本的细菌检测方法至关重要。在这里，开发了一种基于功能二维（2D）金属有机骨架（MOF）纳米酶的电化学探测器，用于灵敏检测致病菌。提出了一种包含万古霉素和抗抗体的双重识别策略，以特异性固定。具有优异过氧化物酶样活性的 2D MOFs 可以有效地催化 -苯二胺生成 2,2-二氨基偶氮苯，这是监测细菌浓度的理想电化学信号读出。在最佳条件下，本生物测定法提供了 10-7.5×10 个菌落形成单位 CFU/mL 的宽检测范围，检测限为 6 CFU/mL，优于大多数先前的报告。此外，建立的电化学传感器可以在存在其他细菌的情况下选择性和准确地识别 。本工作为灵敏和选择性检测 提供了一条新途径，并在临床诊断领域具有广阔的应用前景。
