Zhou Lei, Wu Jun, Ji Jia-Heng, Gao Jie, Liu Yi-Fan, Wang Biao, Yang Shi-Zhong, Gu Ji-Dong, Mu Bo-Zhong
State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China; Engineering Research Center of Microbial Enhanced Oil Recovery, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
Sci Total Environ. 2023 Feb 1;858(Pt 3):159861. doi: 10.1016/j.scitotenv.2022.159861. Epub 2022 Oct 30.
Microbial diversity and activities in petroleum reservoir systems can be altered by water-flooding operation, but the current understanding of the mechanism for such changes in microbial composition characteristics and community is inadequate. In this study, microbial communities especially functional groups in production water from five petroleum reservoirs in China were investigated by chemical and molecular biological analyses. The dominant and core phyla in the five oil reservoirs were Proteobacteria, Deferribacterota, Firmicutes, Desulfobacterota, Euryarchaeota and Thermoplasmatota. At the genus level, the dominant taxa in each petroleum reservoir were different, and not all of the dominant genera were the core members across the five oil reservoirs. The microbiologically influenced corrosion (MIC) were investigated for the functional groups in each production water. The corrosion rates in production water were higher than controls with a positive correlation to the abundances of sulfate-reducing prokaryotes (SRP). The SRP diversity based on the aprA and dsrA gene analysis showed that obvious differences were evident between onshore (JS, SL, DQ and XJ) and offshore (BS) oilfields. The core SRP taxa in onshore oilfields were Desulfomicrobium and Desulfovibrio, also with Desulfotomaculum in medium/low-temperature oil reservoirs (DQ and XJ), but in high-temperature petroleum reservoirs (JS, BS and SL), Archaeoglobus, Thermodesulfobacterium and Thermodesulfovibrio were the core groups. Statistical analysis indicated that temperature, electron acceptors and donors showed significant influence on the SRP community. This research reveals the characteristics of microbial and functional community as well as their interaction mechanism on corrosion in petroleum reservoir environments, and will improve industrial bio-control and management of MIC in oilfields.
注水作业会改变油藏系统中的微生物多样性和活性，但目前对于微生物组成特征和群落发生此类变化的机制了解不足。在本研究中，通过化学和分子生物学分析，对中国五个油藏采出水中的微生物群落尤其是功能菌群进行了调查。五个油藏中的优势菌门和核心菌门为变形菌门、脱铁杆菌门、厚壁菌门、脱硫杆菌门、广古菌门和热原体门。在属水平上，每个油藏中的优势分类单元各不相同，并非所有优势属都是五个油藏中的核心成员。对每个采出水中的功能菌群进行了微生物影响腐蚀（MIC）研究。采出水中的腐蚀速率高于对照，且与硫酸盐还原原核生物（SRP）的丰度呈正相关。基于aprA和dsrA基因分析的SRP多样性表明，陆上（JS、SL、DQ和XJ）和海上（BS）油田之间存在明显差异。陆上油田的核心SRP分类单元为脱硫微菌属和脱硫弧菌属，中/低温油藏（DQ和XJ）中还有脱硫芽孢杆菌属，但在高温油藏（JS、BS和SL）中，嗜热栖热菌属、嗜热脱硫杆菌属和嗜热脱硫弧菌属是核心菌群。统计分析表明，温度、电子受体和供体对SRP群落有显著影响。本研究揭示了油藏环境中微生物和功能群落的特征及其对腐蚀的相互作用机制，将改善油田MIC的工业生物控制和管理。
