Li Xiao-Xiao, Yang Tao, Mbadinga Serge M, Liu Jin-Feng, Yang Shi-Zhong, Gu Ji-Dong, Mu Bo-Zhong
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China.
Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, China.
Front Microbiol. 2017 Dec 5;8:2379. doi: 10.3389/fmicb.2017.02379. eCollection 2017.
Oil reservoir production systems are usually associated with a temperature gradient and oil production facilities frequently suffer from pipeline corrosion failures. Both bacteria and archaea potentially contribute to biocorrosion of the oil production equipment. Here the response of microbial populations from the petroleum reservoir to temperature gradient and corrosion of carbon steel coupons were investigated under laboratory condition. Carbon steel coupons were exposed to production water from a depth of 1809 m of Jiangsu petroleum reservoir (China) and incubated for periods of 160 and 300 days. The incubation temperatures were set at 37, 55, and 65°C to monitoring mesophilic, thermophilic and hyperthermophilic microorganisms associated with anaerobic carbon steel corrosion. The results showed that corrosion rate at 55°C (0.162 ± 0.013 mm year) and 37°C (0.138 ± 0.008 mm year) were higher than that at 65°C (0.105 ± 0.007 mm year), and a dense biofilm was observed on the surface of coupons under all biotic incubations. The microbial community analysis suggests a high frequency of bacterial taxa associated with families Porphyromonadaceae, Enterobacteriaceae, and Spirochaetaceae at all three temperatures. While the majority of known sulfate-reducing bacteria, in particular , and spp., were predominantly observed at 37°C; spp., spp. and spp. as well as archaeal members closely related to and spp. were substantially enriched at 65°C. Hydrogenotrophic methanogens of the family Methanobacteriaceae were dominant at both 37 and 55°C; acetoclastic spp. and methyltrophic spp. were enriched at 37°C. These observations show that temperature changes significantly alter the microbial community structure in production fluids and also affected the biocorrosion of carbon steel under anaerobic conditions.
油藏生产系统通常伴随着温度梯度，石油生产设施经常遭受管道腐蚀故障。细菌和古菌都可能导致石油生产设备的生物腐蚀。在此，在实验室条件下研究了来自油藏的微生物群落对温度梯度和碳钢试片腐蚀的响应。将碳钢试片暴露于中国江苏油藏1809米深处的采出水中，并分别培养160天和300天。培养温度设定为37、55和65°C，以监测与厌氧碳钢腐蚀相关的嗜温、嗜热和超嗜热微生物。结果表明，55°C（0.162±0.013毫米/年）和37°C（0.138±0.008毫米/年）时的腐蚀速率高于65°C（0.105±0.007毫米/年），并且在所有生物培养条件下，试片表面均观察到致密的生物膜。微生物群落分析表明，在所有三个温度下，与卟啉单胞菌科、肠杆菌科和螺旋体科相关的细菌类群出现频率较高。虽然大多数已知的硫酸盐还原菌，特别是 、 和 等菌属，主要在37°C时观察到； 、 和 等菌属以及与 和 等菌属密切相关的古菌成员在65°C时大量富集。甲烷杆菌科的氢营养型产甲烷菌在37和55°C时均占主导地位；乙酸裂解型 等菌属和甲基营养型 等菌属在37°C时富集。这些观察结果表明，温度变化显著改变了采出液中的微生物群落结构，并且在厌氧条件下也影响了碳钢的生物腐蚀。
