Goessens W H, Wouters J T, Fontijne P, Michel M F
J Antimicrob Chemother. 1986 Oct;18(4):459-66. doi: 10.1093/jac/18.4.459.
Cell-bound and extracellular autolytic activity was studied in a tolerant and a non-tolerant Staphylococcus aureus strain after exposure to low (10 mg/l) and high (80 mg/l) concentrations of methicillin. Tolerance was defined as survival after 24 h of over 2% of the inoculum after exposure to high concentrations of methicillin (greater than or equal to 64 mg/l). The nontolerant strain showed an increased cell-bound autolytic activity after exposure to both low and high concentrations of methicillin. The tolerant strain on the other hand selectively showed a reduced cell-bound autolytic activity after exposure to 80 mg/l of methicillin. In both types of strains extracellular activity was less after exposure to a high concentration of methicillin than after exposure to a low concentration. This phenomenon was caused by the release of an inhibitor of the autolysins under the influence of the high concentration of methicillin.
在耐甲氧西林和不耐甲氧西林的金黄色葡萄球菌菌株中，研究了在接触低浓度（10毫克/升）和高浓度（80毫克/升）甲氧西林后细胞结合型和细胞外自溶活性。耐受性定义为在接触高浓度甲氧西林（大于或等于64毫克/升）24小时后接种物存活超过2%。不耐受菌株在接触低浓度和高浓度甲氧西林后，细胞结合型自溶活性均增加。另一方面，耐受菌株在接触80毫克/升甲氧西林后选择性地表现出细胞结合型自溶活性降低。在两种类型的菌株中，接触高浓度甲氧西林后的细胞外活性均低于接触低浓度甲氧西林后的活性。这种现象是由高浓度甲氧西林影响下自溶素抑制剂的释放引起的。
