Food, Nutrition, and Health, Faculty of Land & Food Systems, 2205 East Mall, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Food Chem. 2023 Mar 30;405(Pt B):134600. doi: 10.1016/j.foodchem.2022.134600. Epub 2022 Oct 13.
The effects of Pulsed Light (PL) technology on the anthocyanin condensation reaction in model wine solutions were investigated. Model wine solutions containing malvidin-3-O-glucoside, cyanidin-3-O-glucoside, and delphinidin-3-O-glucoside were separately prepared with the presence of (-)-epicatechin and acetaldehyde. The solutions were subjected to PL treatment with 2, 4, and 8 J/cm energy and stored in 10 °C. The loss of anthocyanin during the treatment and the aging period fitted the first-order reaction model (R > 98 %). Delphinidin-3-O-glucoside suffered the highest loss, only 46 % remaining after 60 s treatment; the malvidin-3-O-glucoside showed the lower loss, 72 % remaining after 60 s treatment. Furthermore, the PL treatment significantly influenced the kinetics of anthocyanin loss. The results from LC ESI TOF/Q-TOF MS/MS analysis revealed that in the PL treated samples, more peaks eluted in the chromatogram assigned to anthocyanin ethyl-linked (-)-epicatechin products, suggesting that PL treatment led to the formation of new isomers of anthocyanin ethyl-linked (-)-epicatechin. The color characteristics of the model solutions were affected by the PL treatment and the formation of ethyl-linked products. For example, the ΔE* value for samples treated with 8 J/cm increased by 42.52, 55.73, and 45.61 % for malvidin-3-O-glucoside, cyanidin-3-O-glucoside, and delphinidin-3-O-glucoside respectively after 110 days.
研究了脉冲光 (PL) 技术对模型酒溶液中花色苷缩合反应的影响。分别用 (-)-表儿茶素和乙醛制备了含有矢车菊素-3-O-葡萄糖苷、飞燕草素-3-O-葡萄糖苷和天竺葵素-3-O-葡萄糖苷的模型酒溶液。将溶液用 2、4 和 8 J/cm 能量进行 PL 处理，并在 10°C 下储存。处理和老化期间花色苷的损失符合一级反应模型（R>98%）。在 60s 处理后，天竺葵素-3-O-葡萄糖苷损失最大，仅残留 46%；在 60s 处理后，矢车菊素-3-O-葡萄糖苷的损失较低，残留 72%。此外，PL 处理显著影响花色苷损失的动力学。LC ESI TOF/Q-TOF MS/MS 分析结果表明，在 PL 处理的样品中，更多的峰在色谱图中被分配到花色苷乙基连接（-）-表儿茶素产物，表明 PL 处理导致花色苷乙基连接（-）-表儿茶素新异构体的形成。模型溶液的颜色特性受到 PL 处理和乙基连接产物形成的影响。例如，在 110 天后，用 8 J/cm 处理的样品的ΔE*值分别增加了 42.52%、55.73%和 45.61%，用于矢车菊素-3-O-葡萄糖苷、飞燕草素-3-O-葡萄糖苷和天竺葵素-3-O-葡萄糖苷。
