光生物调节通过刺激 VEGFR2 和 STAT3 的核转位促进伤口愈合中的血管生成。
Photobiomodulation promotes angiogenesis in wound healing through stimulating the nuclear translocation of VEGFR2 and STAT3.
机构信息
Department of Burn, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
出版信息
J Photochem Photobiol B. 2022 Dec;237:112573. doi: 10.1016/j.jphotobiol.2022.112573. Epub 2022 Sep 22.
In recent years, Photobiomodulation (PBM) has gained prevalence as a kind of physical therapy for wound healing, however, concerning specific cellular mechanisms induced by PBM remains uncertain. The objective of this study is to evaluate the mechanisms of action of PBM (632.8 nm) on angiogenesis in wound healing in vitro and vivo. In the present work, we indicated that PBM with 1.0 J/cm2 irradiation dose exerts positive effects on cell viability, migration, proliferation and tube formation in human umbilical vein endothelial cells (HUVECs). Furthermore, we demonstrate that the VEGFA/VEGFR2/STAT3 pathway plays an important role in PBM effecting cellular function and promoting angiogenesis in wound healing. In addition, we also found that PBM activated the VEGFA/VEGFR2/STAT3 pathway by stimulating VEGFR2 and STAT3 nuclear translocation in the presence of importin-β. Our research offer a new insight into the potential molecular mechanisms in which PBM promotes angiogenesis in wound healing.
近年来，光生物调节（PBM）作为一种物理疗法在伤口愈合中得到了广泛应用，然而，关于 PBM 诱导的具体细胞机制仍不确定。本研究旨在评估 PBM（632.8nm）在体外和体内伤口愈合中对血管生成的作用机制。在本工作中，我们表明，照射剂量为 1.0J/cm2 的 PBM 对人脐静脉内皮细胞（HUVEC）的细胞活力、迁移、增殖和管状结构形成有积极作用。此外，我们证明 VEGFA/VEGFR2/STAT3 通路在 PBM 影响细胞功能和促进伤口愈合中的血管生成中起着重要作用。此外，我们还发现 PBM 通过在存在 Importin-β 的情况下刺激 VEGFR2 和 STAT3 核转位来激活 VEGFA/VEGFR2/STAT3 通路。我们的研究为 PBM 促进伤口愈合中血管生成的潜在分子机制提供了新的见解。
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