Department of Biological Sciences and Chemistry, University of Nizwa, PC 616, Birkat Al-Mouz, 33, Nizwa, Sultanate of Oman.
Research Institute, Medical University Pleven, Sv. Kliment Ohridski Str., 1, Pleven, 5800, Bulgaria.
BMC Gastroenterol. 2022 Nov 21;22(1):477. doi: 10.1186/s12876-022-02562-w.
The epidemiology of Crohn's disease (CD) has changed over the past decades, demonstrating a trend toward increased prevalence in developing countries, while in developed countries, its incidence has stabilized. The study aimed to examine the profile of the key pro-inflammatory cytokines in the serum of patients with CD and establish their association with the severity and activity of the disease.
A total of 61 patients (29 women (47.5%), 32 men (52.5%) aged from 18 to 40 years (mean age (30.42 ± 2.51) years) with the verified diagnosis of CD in the active phase were examined. The control group consisted of 30 healthy people of corresponding age.
CD is characterized by a reliable increase of pro-inflammatory cytokines in blood compared to healthy people: tumor necrosis factor-α (TNF-α) - by 4.45 times (137.46 ± 9.72 vs. 30.88 ± 2.08 pg/ml in healthy people, p < 0,001), interleukin-1α (IL-1α) - by 5.08 times (51.55 ± 4.36 vs. 10.14 ± 0.93 pg/ml, p < 0.001), interleukin-6 (IL-6) - by 2.16 times (20.03 ± 1.81 vs. 9.27 ± 0.52 pg/ml, p < 0.001), interleukin-8 (IL-8) - by 2.04 times (25.74 ± 2.05 vs. 12.62 ± 1.16 pg/ml, p < 0.001), and interferon-γ (IFN-γ) - by 5.30 times (208.63 ± 14.29 vs. 39.35 ± 2.40 pg/ml, p < 0.001). The authors have established direct correlations between the Crohn's disease activity index and blood content of TNF-α (r = 0.84, p < 0.013), INF-γ (r = 0.61, p < 0.028); between TNF-α and INF-γ content (r = 0.67, p < 0.023), IL-1α (r = 0.49, p < 0.042), IL-6 (r = 0.40, p < 0.045), and IL-8 (r = 0.51, p < 0.033); INF-γ and IL-1α (r = 0.53, p < 0.040), IL-6 (r = 0.37, p < 0.039), IL-8 (r = 0.44, p < 0.040).
Patients with CD were found to have multiple cytokines (TNF-α, IL-1α, IL-6, IL-8, and IFN-γ,). The content of cytokines correlated positively with the CD activity index.
过去几十年来，克罗恩病（CD）的流行病学发生了变化，发展中国家的患病率呈上升趋势，而在发达国家，其发病率已趋于稳定。本研究旨在研究 CD 患者血清中关键促炎细胞因子的特征，并确定其与疾病严重程度和活动度的关系。
共检查了 61 名（29 名女性（47.5%），32 名男性（52.5%），年龄在 18 至 40 岁之间（平均年龄（30.42±2.51）岁）确诊为活动期 CD 的患者。对照组由 30 名年龄相匹配的健康人组成。
与健康人相比，CD 患者的血液中促炎细胞因子明显增加：肿瘤坏死因子-α（TNF-α）增加了 4.45 倍（137.46±9.72 vs. 30.88±2.08 pg/ml，p<0.001），白细胞介素-1α（IL-1α）增加了 5.08 倍（51.55±4.36 vs. 10.14±0.93 pg/ml，p<0.001），白细胞介素-6（IL-6）增加了 2.16 倍（20.03±1.81 vs. 9.27±0.52 pg/ml，p<0.001），白细胞介素-8（IL-8）增加了 2.04 倍（25.74±2.05 vs. 12.62±1.16 pg/ml，p<0.001），干扰素-γ（IFN-γ）增加了 5.30 倍（208.63±14.29 vs. 39.35±2.40 pg/ml，p<0.001）。作者发现 CD 活动指数与 TNF-α（r=0.84，p<0.013）、INF-γ（r=0.61，p<0.028）血液含量之间存在直接相关性；TNF-α与 INF-γ（r=0.67，p<0.023）、IL-1α（r=0.49，p<0.042）、IL-6（r=0.40，p<0.045）和 IL-8（r=0.51，p<0.033）之间存在直接相关性；INF-γ与 IL-1α（r=0.53，p<0.040）、IL-6（r=0.37，p<0.039）和 IL-8（r=0.44，p<0.040）之间存在直接相关性。
CD 患者发现有多种细胞因子（TNF-α、IL-1α、IL-6、IL-8 和 IFN-γ）。细胞因子的含量与 CD 活动指数呈正相关。
