de Araújo-Neto Vicente Toscano, Barbosa-Silva Andressa Noronha, Medeiros Honorato Nathan Ravi, Sales Letícia Mikardya Lima, de Cassia Pires Renata, do Nascimento Brito Carlos Ramon, da Matta Guedes Paulo Marcos, da Cunha Galvão Lúcia Maria, da Câmara Antonia Claudia Jácome
Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil.
Graduate Program in Parasitology, Federal University of Minas Gerais, Belo Horizonte, Belo Horizonte, 31270-901, Brazil.
Parasitol Res. 2023 Jan;122(1):207-215. doi: 10.1007/s00436-022-07719-w. Epub 2022 Nov 21.
Trypanosoma cruzi, the etiologic agent of American trypanosomiasis, is a vector-borne zoonotic parasite which has been little studied regarding its infection in domestic animals. In this study, we evaluated the occurrence of natural infection by T. cruzi in farm animals using molecular markers and phylogenetic analysis in blood clot samples of 60 sheep (Ovis aires), 22 goats (Capra hircus), and 14 horses (Equus caballus) in eight municipalities located in an infection risk area in the state of Rio Grande do Norte (RN), Northeast Region of Brazil. Trypanosoma spp. infection was identified by amplifying the rRNA 18S SSU gene in 48.9% of the samples. The SH022 sample showed 99.8% similarity with the Y strain of T. cruzi in phylogeny, grouped in the DTU II clade. Blood clots of sheep, goats, and horses detected T. cruzi kDNA in 28.3% (17/60), 22.7% (5/22), and 15.4% (2/14) of the samples, respectively. These animals were distributed in the three studied mesoregions throughout the state of RN. The identification of natural infection in domestic animals contributes to expand the epidemiological transmission scenario in an area where T. brasiliensis is the main vector.
克氏锥虫是美洲锥虫病的病原体，是一种通过媒介传播的人畜共患寄生虫，关于其在家畜中的感染情况研究较少。在本研究中，我们利用分子标记和系统发育分析，对巴西北部地区北大河州（RN）一个感染风险区域内八个市的60只绵羊（Ovis aires）、22只山羊（Capra hircus）和14匹马（Equus caballus）的血凝块样本进行检测，评估家畜中克氏锥虫自然感染的发生情况。通过扩增rRNA 18S SSU基因，在48.9%的样本中鉴定出锥虫属感染。SH022样本在系统发育上与克氏锥虫Y株显示出99.8%的相似性，归为DTU II进化枝。绵羊、山羊和马的血凝块样本中，分别有28.3%（17/60）、22.7%（5/22）和15.4%（2/14）检测到克氏锥虫kDNA。这些动物分布在RN州的三个研究中观区域。家畜中自然感染的鉴定有助于扩大以巴西锥蝽为主要传播媒介地区的流行病学传播情况。
