Vellapandian Chitra, R Rini, Ramachandram Dinesh Sangarran
Department Of Pharmacology, SRM College Of Pharmacy, SRM Institute of Science and Technology, 603203 Kattankulathur, Chengalpet, India.
Monash School of Pharmacy, Monash University, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor Darul Ehsan, Monash, Malaysia.
J Diabetes Metab Disord. 2022 Aug 12;21(2):1557-1567. doi: 10.1007/s40200-022-01101-4. eCollection 2022 Dec.
This study was carried out to study the effect of anethole rich fraction on the Diabetic Nephropathy (DN) rats, and explore the mechanisms.
Male wistar rats were grouped into 4 (n = 6 per group): Control, junk food fed DN induced rats, low dose and high dose groups. DN was induced by oral junk food feeding. They were monitored for blood glucose levels and urine protein content at regular intervals. At the end of the study, the biological and hematological parameters were measured. Pancreatic and kidney viscera were taken to observe histopathological changes.
Both the doses of anethole rich fraction could drastically lower blood glucose levels, Low Density Lipoprotein (LDL), Glycated Serum Protein (GSP), Total Cholesterol (TC), Triglycerides (TG) (P < 0.01), Malondialdehyde (MDA) level (P < 0.01 or P < 0.05), increases insulin level (P < 0.01), High Density Lipoproteins (HDL), Glutathione Reductase (GSH) and Superoxide Dismutase (SOD) (P < 0.01 or P < 0.05 or P > 0.05). Both doses of anethole rich fraction also improved the pathological changes of kidney and pancreatic tissues in DN rats (P < 0.01 or P < 0.05 or P > 0.05).
Hence it can be hypothesized that the high dose of anethole rich fraction (100 mg/kg) could reach the intervention effect and could ameliorate renal damage in DN rats by improving the renal functions, oxidative stress levels, glycometabolism and pathological changes of pancreas and kidney.
本研究旨在探讨富含茴香脑的提取物对糖尿病肾病（DN）大鼠的影响，并探究其作用机制。
将雄性Wistar大鼠分为4组（每组n = 6）：对照组、喂食垃圾食品诱导的DN大鼠组、低剂量组和高剂量组。通过口服垃圾食品诱导糖尿病肾病。定期监测大鼠的血糖水平和尿蛋白含量。在研究结束时，测量生物学和血液学参数。摘取胰腺和肾脏脏器，观察组织病理学变化。
富含茴香脑的提取物的两个剂量均可显著降低血糖水平、低密度脂蛋白（LDL）、糖化血清蛋白（GSP）、总胆固醇（TC）、甘油三酯（TG）（P < 0.01）、丙二醛（MDA）水平（P < 0.01或P < 0.05），提高胰岛素水平（P < 0.01）、高密度脂蛋白（HDL）、谷胱甘肽还原酶（GSH）和超氧化物歧化酶（SOD）（P < 0.01或P < 0.05或P > 0.05）。富含茴香脑的提取物的两个剂量还改善了DN大鼠肾脏和胰腺组织的病理变化（P < 0.01或P < 0.05或P > 0.05）。
因此，可以推测高剂量的富含茴香脑的提取物（100 mg/kg）可达到干预效果，并通过改善肾功能、氧化应激水平、糖代谢以及胰腺和肾脏的病理变化来减轻DN大鼠的肾损伤。
