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在 L. 中挖掘与雌蕊败育相关的基因

Mining for genes related to pistil abortion in L.

机构信息

College of Forestry, Shenyang Agricultural University, Shenyang, China.

出版信息

PeerJ. 2022 Nov 15;10:e14366. doi: 10.7717/peerj.14366. eCollection 2022.

DOI:10.7717/peerj.14366
PMID:36405023
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9673769/
Abstract

In , the phenomenon of pistil abortion is very common and seriously affects its fruit quality and yield; however, the molecular mechanisms of pistil abortion remains unclear. In this study, we identified differentially expressed genes (DEGs) and pathways associated with pistil abortion using transcriptome sequencing. After comparative analysis, a total of 1,950 DEGs were identified, of which 1,000 were upregulated, and 950 were downregulated. Gene Ontology (GO) functional enrichment analysis of DEGs showed that metabolic process, cellular process, single-organism process, membrane, membrane part, cell, binding, catalytic activity, and transporter activity contained the largest number of DEGs. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the plant-pathogen interaction, starch and sucrose metabolism, and plant hormone signal transduction pathways contained the largest number of DEGs. The NAC, bHLH, and B3 transcription factor families contained the largest number of DEGs. qRT-PCR detection confirmed that the gene expression levels were consistent with the transcriptome sequencing results. This study provides a theoretical basis and scientific basis for further research on the molecular mechanisms of pistil abortion.

摘要

在 中，柱头败育现象非常普遍，严重影响其果实品质和产量；然而，柱头败育的分子机制尚不清楚。在这项研究中，我们使用转录组测序鉴定了与柱头败育相关的差异表达基因（DEGs）和途径。经过比较分析，共鉴定出 1950 个 DEGs，其中 1000 个上调，950 个下调。DEGs 的基因本体论（GO）功能富集分析表明，代谢过程、细胞过程、单个生物体过程、膜、膜部分、细胞、结合、催化活性和转运活性包含最多的 DEGs。京都基因与基因组百科全书（KEGG）途径富集分析表明，植物-病原体相互作用、淀粉和蔗糖代谢以及植物激素信号转导途径包含最多的 DEGs。NAC、bHLH 和 B3 转录因子家族包含最多的 DEGs。qRT-PCR 检测证实基因表达水平与转录组测序结果一致。本研究为进一步研究柱头败育的分子机制提供了理论依据和科学基础。

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