检测和鉴定泰国北部河水中产 ESBL 菌和其他共存基因。

Detection and characterization of ESBL-producing and additional co-existence with genes from river water in northern Thailand.

机构信息

School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand.

Division of Hematopoiesis, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Kumamoto, Japan.

出版信息

PeerJ. 2022 Nov 14;10:e14408. doi: 10.7717/peerj.14408. eCollection 2022.

BACKGROUND

Extended-spectrum β-lactamase producing (ESBL-producing ) have emerged, causing human and animal infections worldwide. This study was conducted to investigate the prevalence and molecular genetic features of ESBL-producing and multidrug-resistant (MDR) in river water.

METHODS

A total of 172 samples were collected from the Kok River and Kham River in Chiang Rai, Thailand, during a 10-month period (2020-2021).

RESULTS

We detected 45.3% of to be MDR. The prevalence of ESBL-producers was 22%. Among those ESBL-producing strains, CTX-M-15 (44.7%) was predominantly found, followed by CTX-M-55 (26.3%), CTX-M-14 (18.4%), and CTX-M-27 (10.5%). The and genes were found to be co-harbored with the genes. Mobile elements, ., IS1 and Tn, were observed. Twelve plasmid replicons were found, predominantly being IncF (76.3%) and IncFIB (52.6%). Whole genome sequencing of ten selected isolates revealed the co-existence of ESBL with genes in two ESBL-producing . A wide diversity of MLST classifications was observed. An -- gene cassette was found to disrupt the PUF2806 domain-containing gene, while an contig on another isolate contained the /-- core segment.

DISCUSSION

In conclusion, our data provides compelling evidence of MDR and ESBL-producing , co-existing with genes in river water in northern Thailand, which may be disseminated into other environments and so cause increased risks to public health.

背景

产生超广谱β-内酰胺酶（ESBL 产生）的已经出现，导致全球人类和动物感染。本研究旨在调查河流水中 ESBL 产生和多药耐药（MDR）的流行情况和分子遗传特征。

方法

在 2020 年至 2021 年期间，从泰国清莱的孔河和克姆河共采集了 172 个样本。

结果

我们发现 45.3%的为 MDR。ESBL 产生菌的检出率为 22%。在这些 ESBL 产生菌中，主要发现 CTX-M-15（44.7%），其次是 CTX-M-55（26.3%）、CTX-M-14（18.4%）和 CTX-M-27（10.5%）。和基因与基因共同存在。观察到移动元件、. 、IS1 和 Tn。发现了 12 种质粒复制子，主要为 IncF（76.3%）和 IncFIB（52.6%）。对 10 个选定分离株进行全基因组测序，发现两种 ESBL 产生菌中 ESBL 与基因共存。观察到多种 MLST 分类。在两个 ESBL 产生菌中，发现一个基因盒破坏了 PUF2806 结构域基因，而另一个分离株的基因片段则包含 /--核心片段。