Wen Yi, Liu Wenjie, David Benjamin, Ren Wen, Irudayaraj Joseph
Department of Bioengineering, College of Engineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Biomedical Research Center, Mills Breast Cancer Institute, Carle Foundation Hospital, Urbana, Illinois 61801, United States.
ACS Omega. 2022 Nov 1;7(45):41275-41283. doi: 10.1021/acsomega.2c04953. eCollection 2022 Nov 15.
We propose a novel multifunctional nanocarrier system for targeted drug delivery for lung cancer theranostics. Oxygenated particles (OPs) synthesized with an oxygen-encapsulating carboxymethyl cellulose shell were used as a platform to deliver oxygen to the hypoxic tumor microenvironment. The OPs synthesized could also be conjugated with ligands (e.g., antibodies) to target cancer cells expressing the corresponding antigens to deliver a drug, doxorubicin. In vitro testing of functionalized OPs showed increased efficacy of doxorubicin against the proliferation of lung cancer cells. Both confocal fluorescence imaging and darkfield microscopy hyperspectral imaging validated the OP complex and its efficient targeting of specific cells to deliver the therapeutic. The nanocarrier platform developed can also serve as a diagnostic imaging reagent as demonstrated by darkfield microscopy. Results show that the theranostic OPs developed with multifunctional modalities enabled targeted drug delivery with improved efficacy and tracking of drug delivery vehicles by imaging.
我们提出了一种新型多功能纳米载体系统，用于肺癌诊疗的靶向药物递送。用包裹氧气的羧甲基纤维素壳合成的充氧颗粒（OPs）被用作向缺氧肿瘤微环境输送氧气的平台。合成的OPs还可以与配体（如抗体）偶联，以靶向表达相应抗原的癌细胞来递送药物阿霉素。功能化OPs的体外测试表明，阿霉素对肺癌细胞增殖的疗效增强。共聚焦荧光成像和暗场显微镜高光谱成像均验证了OP复合物及其对特定细胞的有效靶向递送治疗剂。如暗场显微镜所示，开发的纳米载体平台还可以用作诊断成像试剂。结果表明，具有多种功能模式的诊疗OPs能够实现靶向药物递送，提高疗效，并通过成像跟踪药物递送载体。
