Basim Yalda, Mohebali Ghasemali, Jorfi Sahand, Nabizadeh Ramin, Moghadam Mehdi Ahmadi, Ghadiri Ata, Haghighi Fard Nematollah Jaafarzadeh
Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Microbiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, Iran.
J Environ Health Sci Eng. 2022 Jul 6;20(2):601-608. doi: 10.1007/s40201-020-00592-8. eCollection 2022 Dec.
The main purpose of this research was investigating of bioremediation potential oily contaminated soils using native bacterial strains in an oil field.
In this research, total bacterial consortium were identified in oily soils with sandy loam texture as case and non-contaminated soils as controls during six months. The dominant strains present on contaminated soil were identified by DNA extraction using 16S rDNA gene sequencing via NGS technique and compared with bacteria present in non-contaminated soil as control samples. Furthermore, quantitative variations of bacterial count along with total petroleum hydrocarbons (TPH) removal was performed in oily (case) samples to investigate the relation between TPH removal and changes in bacterial density. The TPH values were determined with gas chromatography equipped with a flame ionization detector (GC-FID).
The dominant identified bacteria in oily soil were as follows: which varied significantly from those identified in control soil. The bacterial diversity was higher in contaminated soil and a TPH removal of 50.9% was observed over a period of six months monitoring.
Indigenous bacteria in oil-contaminated soils of an oilfield in south west of Iran were found to be able to degrade Total Petroleum Hydrocarbons. Our results showed that bioremediation of oil-contaminated soils can be implemented without need to amplification of heterogeneous bacteria. Considering sandy loam texture of soil samples, the identified strains of bacteria could be introduced as sufficient consortium for biodegradation of this soils with similar texture.
本研究的主要目的是调查利用油田中的本地细菌菌株对受油污染土壤进行生物修复的潜力。
在本研究中，以砂壤土质地的含油土壤为案例，未受污染的土壤为对照，在六个月的时间里对总细菌群落进行了鉴定。通过使用16S rDNA基因测序的NGS技术提取DNA，鉴定污染土壤中存在的优势菌株，并与作为对照样品的未受污染土壤中存在的细菌进行比较。此外，在含油（案例）样品中进行细菌数量的定量变化以及总石油烃（TPH）去除情况的研究，以探讨TPH去除与细菌密度变化之间的关系。TPH值通过配备火焰离子化检测器（GC-FID）的气相色谱法测定。
在含油土壤中鉴定出的优势细菌如下：与对照土壤中鉴定出的细菌有显著差异。污染土壤中的细菌多样性更高，在六个月的监测期内观察到TPH去除率为50.9%。
发现在伊朗西南部一个油田的受油污染土壤中的本土细菌能够降解总石油烃。我们的结果表明，无需扩增异质细菌就可以对受油污染土壤进行生物修复。考虑到土壤样品的砂壤土质地，所鉴定出的细菌菌株可以作为对具有相似质地的该类土壤进行生物降解的足够群落引入。
