National Centre of Excellence in Geology, University of Peshawar, Peshawar 25130, Pakistan.
Department of Environmental Sciences, University of Swabi, Swabi 23561, Pakistan.
Molecules. 2022 Mar 17;27(6):1945. doi: 10.3390/molecules27061945.
Nano-bioremediation application is an ecologically and environmentally friendly technique to overcome the catastrophic situation in soil because of petroleum waste contamination. We evaluated the efficiency of oil-degrading bacterial consortium and silver nanoparticles (AgNPs) with or without fertilizer to remediate soils collected from petroleum waste contaminated oil fields. Physicochemical characteristics of control soil and petroleum contaminated soils were assessed. Four oil-degrading strains, namely (KY010576), (KY010578), (KY010586), and (KX580766), were selected based on their in vitrohydrocarbon-degrading efficiency. In a lab experiment, contaminated soils were treated alone and with combined amendments of the bacterial consortium, AgNPs, and fertilizers (ammonium nitrate and diammonium phosphate). We detected the degradation rate of total petroleum hydrocarbons (TPHs) of the soil samples with GC-FID at different intervals of the incubation period (0, 5, 20, 60, 240 days). The bacterial population (CFU/g) was also monitored during the entire period of incubation. The results showed that 70% more TPH was degraded with a consortium with their sole application in 20 days of incubation. There was a positive correlation between TPH degradation and the 100-fold increase in bacterial population in contaminated soils. This study revealed that bacterial consortiums alone showed the maximum increase in the degradation of TPHs at 20 days. The application of nanoparticles and fertilizer has non-significant effects on the consortium degradation potential. Moreover, fertilizer alone or in combination with AgNPs and consortium slows the rate of degradation of TPHs over a short period. Still, it subsequently accelerates the rate of degradation of TPHs, and a negligible amount remains at the end of the incubation period.
纳米生物修复应用是一种生态和环境友好的技术，可以克服因石油废物污染而导致的土壤灾难性情况。我们评估了石油降解细菌群落和银纳米粒子 (AgNPs) 单独或与肥料一起修复从受石油废物污染的油田采集的土壤的效率。评估了对照土壤和受石油污染土壤的理化特性。根据体外烃降解效率，选择了四种石油降解菌株，即 （KY010576）、 （KY010578）、 （KY010586）和 （KX580766）。在实验室实验中，单独处理和联合添加细菌群落、AgNPs 和肥料（硝酸铵和磷酸二铵）处理污染土壤。我们使用 GC-FID 在不同的孵育期（0、5、20、60、240 天）间隔检测土壤样品中总石油烃 (TPHs) 的降解率。在整个孵育期也监测了细菌种群 (CFU/g)。结果表明，在 20 天的孵育期内，单独使用群落可使 TPH 降解 70%。在受污染土壤中，TPH 降解与细菌种群增加 100 倍之间存在正相关。本研究表明，细菌群落单独在 20 天内显示出 TPHs 最大降解增加。纳米粒子和肥料的单独或联合应用对群落的降解潜力没有显著影响。此外，肥料单独或与 AgNPs 和群落联合在短时间内会降低 TPHs 的降解速率，但随后会加速 TPHs 的降解速率，在孵育期结束时几乎没有剩余。
