Pandey Rakesh, Mani Dayanandan, Shanker Karuna, Bawankule Dnyaneshwar Umrao, Chanda Debabrata, Lal Raj Kishori, Pal Anirban, Khare Puja, Kumar Narendra, Tandon Sudeep, Saikia Dharmendra, Gupta Anil Kumar, Srivastava Ramesh Kumar, Kumar Sanjay, Suresh Ram, Singh Saudan, Kalra Alok, Maurya Anil, Singh Dewasya Pratap, Pandey Taruna, Trivedi Shalini, Smita Shachi Suchi, Pant Akanksha, Rathor Laxmi, Asthana Jyotsna, Trivedi Mashu, Trivedi Prabodh Kumar
CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015 India.
Nucleus (Calcutta). 2022;65(3):303-320. doi: 10.1007/s13237-022-00407-1. Epub 2022 Nov 10.
The positive effect of herbal supplements on aging and age-related disorders has led to the evolution of natural curatives for remedial neurodegenerative diseases in humans. The advancement in aging is exceedingly linked to oxidative stress. Enhanced oxidative stress interrupts health of humans in various ways, necessitating to find stress alleviating herbal resources. Currently, minimal scientifically validated health and cognitive booster resources are available. Therefore, we explored the impact of plant extracts in different combinations on oxidative stress, life span and cognition using the multicellular transgenic humanized , and further validated the same in , besides testing their safety and toxicity. In our investigations, the final product-the HACBF (healthy ageing cognitive booster formulation) thus developed was found to reduce major aging biomarkers like lipofuscin, protein carbonyl, lipid levels and enhanced activity of antioxidant enzymes. Further confirmation was done using transgenic worms and RT-PCR. The cognitive boosting activities analyzed in and model system were found to be at par with donepezil and L-dopa, the two drugs which are commonly used to treat Parkinson's and Alzheimer's diseases. In the transgenic model system, the HACBF exhibited reduced aggregation of misfolded disease proteins α-synuclein and increased the health of nicotinic acetylcholine receptor, levels of Acetylcholine and Dopamine contents respectively, the major neurotransmitters responsible for memory, language, learning behavior and movement. Molecular studies clearly indicate that HACBF upregulated major genes responsible for healthy aging and cognitive booster activities in and as well as in . As such, the present herbal product thus developed may be quite useful for healthy aging and cognitive boosting activities, and more so during this covid-19 pandemic.
The online version contains supplementary material available at 10.1007/s13237-022-00407-1.
草药补充剂对衰老及与年龄相关疾病的积极作用，促使了用于治疗人类神经退行性疾病的天然疗法的发展。衰老进程与氧化应激密切相关。氧化应激增强会以多种方式损害人类健康，因此有必要寻找能减轻应激的草药资源。目前，经过科学验证的健康和认知增强资源极少。因此，我们利用多细胞转基因人源化模型，探究了不同组合的植物提取物对氧化应激、寿命和认知的影响，并在另外的模型中进行了验证，同时测试了它们的安全性和毒性。在我们的研究中，最终开发出的产品——健康衰老认知增强配方（HACBF），被发现可降低主要的衰老生物标志物水平，如脂褐素、蛋白质羰基、脂质水平，并增强抗氧化酶的活性。通过转基因蠕虫和逆转录聚合酶链反应（RT-PCR）进行了进一步验证。在果蝇和线虫模型系统中分析发现，HACBF的认知增强活性与多奈哌齐和左旋多巴相当，这两种药物常用于治疗帕金森病和阿尔茨海默病。在转基因线虫模型系统中，HACBF减少了错误折叠的疾病蛋白α-突触核蛋白的聚集，并分别提高了烟碱型乙酰胆碱受体的健康水平、乙酰胆碱和多巴胺含量，这两种是负责记忆、语言、学习行为和运动的主要神经递质。分子研究清楚地表明，HACBF上调了在果蝇和线虫以及其他模型中负责健康衰老和认知增强活动的主要基因。因此，目前开发的这种草药产品可能对健康衰老和认知增强活动非常有用，在当前的新冠疫情期间更是如此。
在线版本包含可在10.1007/s13237-022-00407-1获取的补充材料。
