Zhang YanYing, Shen Tao, Zuo ZhiTian, Wang YuanZhong
Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.
College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, China.
Front Plant Sci. 2022 Nov 2;13:996069. doi: 10.3389/fpls.2022.996069. eCollection 2022.
As a fungus with both medicinal and edible value, (F. A. Wolf) Ryvarden & Gilb. has drawn more public attention. Chemical components' content fluctuates in wild and cultivated , whereas the accumulation ability of chemical components in different parts is different. In order to perform a quality assessment of , we proposed a comprehensive method which was mainly realized by Fourier transform near-infrared (FT-NIR) spectroscopy and ultra-fast liquid chromatography (UFLC). A qualitative analysis means was built a residual convolutional neural network (ResNet) to recognize synchronous two-dimensional correlation spectroscopy (2DCOS) images. It can rapidly identify samples from wild and cultivated in different parts. As a quantitative analysis method, UFLC was used to determine the contents of three triterpene acids in 547 samples. The results showed that a simultaneous qualitative and quantitative strategy could accurately evaluate the quality of . The accuracy of ResNet models combined synchronous FT-NIR 2DCOS in identifying wild and cultivated in different parts was as high as 100%. The contents of three triterpene acids in Poriae Cutis were higher than that in Poria, and the one with wild Poriae Cutis was the highest. In addition, the suitable habitat plays a crucial role in the quality of . The maximum entropy (MaxEnt) model is a common method to predict the suitable habitat area for under the current climate. Through the results, we found that suitable habitats were mostly situated in Yunnan Province of China, which accounted for approximately 49% of the total suitable habitat area of China. The research results not only pave the way for the rational planting in Yunnan Province of China and resource utilization of , but also provide a basis for quality assessment of medicinal fungi.
作为一种具有药用和食用价值的真菌,茯苓(F. A. Wolf)Ryvarden & Gilb. 已引起了更多公众关注。野生茯苓和栽培茯苓中化学成分的含量存在波动,且不同部位化学成分的积累能力也有所不同。为了对茯苓进行质量评估,我们提出了一种主要通过傅里叶变换近红外(FT-NIR)光谱和超快速液相色谱(UFLC)实现的综合方法。构建了一种基于残差卷积神经网络(ResNet)的定性分析手段来识别同步二维相关光谱(2DCOS)图像。它能够快速识别不同部位野生茯苓和栽培茯苓的样本。作为一种定量分析方法,UFLC用于测定547个样本中三种三萜酸的含量。结果表明,定性和定量同步策略能够准确评估茯苓的质量。结合同步FT-NIR 2DCOS的ResNet模型在识别不同部位野生茯苓和栽培茯苓方面的准确率高达100%。茯苓皮中三种三萜酸的含量高于茯苓,其中野生茯苓皮中的含量最高。此外,适宜的生境对茯苓的质量起着至关重要的作用。最大熵(MaxEnt)模型是预测当前气候条件下茯苓适宜生境面积的常用方法。通过结果我们发现,适宜生境大多位于中国云南省,约占中国适宜生境总面积的49%。研究结果不仅为中国云南省茯苓的合理种植和资源利用铺平了道路,也为药用真菌的质量评估提供了依据。