胶原诱导关节炎大鼠中 microRNA 的表达和功能分析。
Expression and function analysis of microRNA in collagen-induced arthritis rats.
机构信息
Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008.
Department of Management Science and Engineering, Business School, Central South University, Changsha 410083, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2022 Sep 28;47(9):1208-1216. doi: 10.11817/j.issn.1672-7347.2022.210789.
OBJECTIVES
Rheumatoid arthritis is a common autoimmune disease, and microRNAs (miRNAs) are involved in its pathogenesis. This study aims to examine the differentially expressed miRNAs in collagen-induced arthritis (CIA) rats, to analyze the biological functions and the related pathways of the miRNA target genes.
METHODS
The total RNA in the synovium of experimental animals was extracted. The miRNA gene profile was obtained by miRNA microarray. Then the differentially expressed miRNAs were screened and the relevant target mRNAs were predicted. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed on the significantly differentially expressed miRNAs.
RESULTS
There were 69 differentially expressed miRNAs including rno-miR-6215 and rno-miR-709 in CIA rats, of which 22 (31.9%) were up-regulated and 47 (68.1%) were down-regulated. GO and KEGG enrichment analysis showed that the up-regulated miRNA target genes were mainly enriched in cellular metabolism, and they were involved in MAPK and Wnt signaling pathways. The down-regulated miRNA target genes were mainly enriched in nervous system development, and they were involved in axon guidance signaling pathway.
CONCLUSIONS
There are differentially expressed miRNAs in the CIA rat model, which may be involved in metabolism biological functions and signal pathways such as MAPK and Wnt.
目的
类风湿关节炎是一种常见的自身免疫性疾病，microRNAs（miRNAs）参与其发病机制。本研究旨在检测胶原诱导关节炎（CIA）大鼠中差异表达的 miRNAs，分析 miRNA 靶基因的生物学功能和相关通路。
方法
提取实验动物滑膜中的总 RNA。通过 miRNA 微阵列获得 miRNA 基因谱。然后筛选差异表达的 miRNAs，并预测相关靶 mRNAs。对显著差异表达的 miRNAs 进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析。
结果
CIA 大鼠中有 69 个差异表达的 miRNAs，包括 rno-miR-6215 和 rno-miR-709，其中 22 个（31.9%）上调，47 个（68.1%）下调。GO 和 KEGG 富集分析表明，上调的 miRNA 靶基因主要富集在细胞代谢中，它们参与 MAPK 和 Wnt 信号通路。下调的 miRNA 靶基因主要富集在神经系统发育中，它们参与轴突导向信号通路。
结论
CIA 大鼠模型中存在差异表达的 miRNAs，可能参与代谢等生物学功能和 MAPK、Wnt 等信号通路。
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