Atkinson J P, Weiss A, Ito M, Kelly J, Parker C W
J Cyclic Nucleotide Res. 1979;5(2):107-23.
L-ascorbic acid (LAA) augmented cGMP many-fold in highly purified human peripheral blood lymphocytes. The cGMP response occurred within 10 sec and persisted for at least 60 min. D-ascorbic acid (DAA) and dehydroascorbic acid (DHAA) were also equally active in enhancing cGMP concentrations but metabolic precursors of ascorbic acid and other inorganic acids did not increase cGMP levels. Determination of the amount of DHAA contaminating the LAA precluded the possibility that it was solely responsible for the enhanced cGMP levels. The sodium or calcium salts of ascorbic acid did not increase cGMP concentrations. If these neutralized preparations were acidified, increased cGMP concentrations were then noted. In broken cell preparations, LAA, DAA, and DHAA and to a lesser extent sodium ascorbate (NaA) enhanced guanylate cyclase activity while neither inhibited cAMP or cGMP phosphodiesterase (PDE) activity. The possible role of H2O2, fatty acid liberation, prostaglandin production, oxidizing-reducing agents, and free radical formation in mediating the effects of ascorbic acid on cGMP levels were evaluated, but none of these potential mechanisms were definitively proven to be a required intermediary for the cGMP enhancing activity of ascorbic acid. LAA, DHAA or NaA did not induce lymphocyte transformation or modulate lectin-induced mitogenesis.
L-抗坏血酸（LAA）可使高度纯化的人外周血淋巴细胞中的环鸟苷酸（cGMP）增加数倍。cGMP反应在10秒内出现，并持续至少60分钟。D-抗坏血酸（DAA）和脱氢抗坏血酸（DHAA）在提高cGMP浓度方面也同样有效，但抗坏血酸的代谢前体和其他无机酸不会增加cGMP水平。对LAA中污染的DHAA量的测定排除了其单独导致cGMP水平升高的可能性。抗坏血酸的钠盐或钙盐不会增加cGMP浓度。如果将这些中和制剂酸化，则会观察到cGMP浓度增加。在破碎细胞制剂中，LAA、DAA和DHAA以及程度较轻的抗坏血酸钠（NaA）增强了鸟苷酸环化酶活性，而既不抑制环磷酸腺苷（cAMP）也不抑制cGMP磷酸二酯酶（PDE）活性。评估了过氧化氢、脂肪酸释放、前列腺素产生、氧化还原剂和自由基形成在介导抗坏血酸对cGMP水平影响中的可能作用，但这些潜在机制均未被明确证明是抗坏血酸cGMP增强活性所需的中介物。LAA、DHAA或NaA不会诱导淋巴细胞转化或调节凝集素诱导的有丝分裂。
