Lemos Vanessa P A, Porto Michele, Cezar Rafael DA S, Santos Bruno P Dos, Souza Melissa R DE, Silva Juliana DA, Nardi Nance B, Camassola Melissa
Universidade Luterana do Brasil, Laboratório de Células-tronco e Engenharia de Tecidos, Av. Farroupilha, 8001, Prédio 22, 5º andar, São José, 92425-020 Canoas, RS, Brazil.
Laboratory for Tissue Engineering - BioTis Inserm U1026, University of Bordeaux, Bât. 4A, 2ème étage, Porte 5, 146 rue Léo Saignat, Bordeaux cedex, France.
An Acad Bras Cienc. 2022 Nov 21;94(suppl 3):e20211246. doi: 10.1590/0001-3765202220211246. eCollection 2022.
Mesenchymal stem cells present clinical potential to recover and regenerate injured tissues in diverse pathologies. The in vitro expansion and characterization of these cells contribute to elucidation of the mechanisms of senescence and strategies involving cell therapies. This study aimed to compare specific characteristics between initial and advanced passages of mesenchymal stem cells derived from adipose tissue and bone marrow. Both cell types were characterized according to immunophenotype, osteogenic differentiation, genomic instability, migration assay, doubling population time and colony forming ability. Our results demonstrated that both cell types were able to maintain an immunophenotypic profile typical of mesenchymal stem cells during increasing passages. Adipose stem cells at initial passage presented greater migration capacity compared to advanced passage cells, and advanced passage cells proliferated faster than initial passage cells. Bone marrow stem cells at early passages presented higher osteogenic potential than advanced. At advanced passages they presented higher colony forming capacity and genetic damage than those at initial passage. These results suggest that mesenchymal stem cells maintained in culture presented characteristics of senescence that should be monitored prior the use in regenerative medicine and cells derived from bone marrow at initial passage have better potential for therapeutic use in bone tissue engineering.
间充质干细胞在多种病理情况下具有恢复和再生受损组织的临床潜力。这些细胞的体外扩增和特性研究有助于阐明衰老机制以及涉及细胞治疗的策略。本研究旨在比较源自脂肪组织和骨髓的间充质干细胞初始传代和后期传代之间的特定特性。两种细胞类型均根据免疫表型、成骨分化、基因组不稳定性、迁移试验、群体倍增时间和集落形成能力进行表征。我们的结果表明，在传代增加过程中，两种细胞类型均能够维持间充质干细胞典型的免疫表型特征。与后期传代细胞相比，初始传代的脂肪干细胞具有更大的迁移能力，且后期传代细胞的增殖速度比初始传代细胞快。早期传代的骨髓干细胞比后期传代具有更高的成骨潜力。在后期传代时，它们比初始传代具有更高的集落形成能力和遗传损伤。这些结果表明，培养的间充质干细胞呈现衰老特征，在用于再生医学之前应进行监测，并且初始传代的骨髓来源细胞在骨组织工程中具有更好的治疗应用潜力。
