Department of Neurosurgery, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, China.
Hospital chief office, Tailai people's Hospital, Qiqihar, China.
World J Surg Oncol. 2022 Nov 23;20(1):368. doi: 10.1186/s12957-022-02831-8.
This study aimed to investigate FAM84B expression in glioma tissues and explore the role of FAM84B in promoting the proliferation of glioma cells and the mechanism of regulating the cell cycle pathways.
The TCGA database was adopted to analyze FAM84B expression in glioma tissues. The FAM84B expression was detected by qRT-PCR in patients with glioma, especially that in glioma cells, U251, LN-229, U98, and U87. Two glioma cell lines U87 and T98 were selected for siRNA transfection, which were divided into si-NC si-FAM84B-1 and si-FAM84B-2 groups. The effect of FAM84B on the proliferation of glioma cells was detected with the MTT experiment and that on the glioma cell cycle was detected with the flow cytometry. The signaling pathways potentially regulated by FAM84B in glioma were analyzed through the bioinformatics analysis. The expression of proteins, Cyclin D1, CDK4, Cdk6, and p21, in the cell cycle-related pathways in cells of each group was detected by the Western blot.
TCGA database results showed a significantly higher FAM84B expression in glioma tissues than that in paracancerous tissues. According to the detection of qRT-PCR, FAM84B expressed the highest in the glioma cell line U87 (P < 0.05). Compared with the serum of healthy controls, FAM84B mRNA expression significantly increased in patients with gliomas. And compared with the si-NC group, the proliferation ability of U87 and T98 cells decreased and the cell cycle was blocked in the G0/G1 phase in both si-FAM84B transfection groups (P < 0.05). According to the bioinformatics analysis, FAM84B regulated the cell cycle pathways in glioma. FAM84B siRNA inhibited the expression of key proteins, Cyclin D1, CDK2, CDK4, and Cdk6, of the cell cycle pathways in glioma cells and promoted the expression of P53 and P21 proteins.
In conclusion, FAM84B may inhibit the proliferation of glioma cells by regulating the cell cycle pathways.
本研究旨在探讨 FAM84B 在脑胶质瘤组织中的表达，并探索 FAM84B 促进脑胶质瘤细胞增殖的作用及其调控细胞周期通路的机制。
采用 TCGA 数据库分析脑胶质瘤组织中 FAM84B 的表达。采用 qRT-PCR 检测脑胶质瘤患者，尤其是脑胶质瘤细胞系 U251、LN-229、U98 和 U87 中 FAM84B 的表达。选择脑胶质瘤细胞系 U87 和 T98 进行 siRNA 转染，分为 si-NC、si-FAM84B-1 和 si-FAM84B-2 组。MTT 实验检测 FAM84B 对脑胶质瘤细胞增殖的影响，流式细胞术检测 FAM84B 对脑胶质瘤细胞周期的影响。通过生物信息学分析分析 FAM84B 可能调控的脑胶质瘤信号通路。Western blot 检测各组细胞周期相关通路中蛋白 Cyclin D1、CDK4、Cdk6 和 p21 的表达。
TCGA 数据库结果显示，脑胶质瘤组织中 FAM84B 的表达明显高于癌旁组织。根据 qRT-PCR 检测，FAM84B 在脑胶质瘤细胞系 U87 中表达最高（P<0.05）。与健康对照者的血清相比，脑胶质瘤患者的 FAM84B mRNA 表达显著增加。与 si-NC 组相比，si-FAM84B 转染组 U87 和 T98 细胞的增殖能力降低，细胞周期阻滞于 G0/G1 期（P<0.05）。生物信息学分析显示，FAM84B 调控脑胶质瘤细胞周期通路。FAM84B siRNA 抑制脑胶质瘤细胞周期通路关键蛋白 Cyclin D1、CDK2、CDK4 和 Cdk6 的表达，促进 P53 和 P21 蛋白的表达。
总之，FAM84B 可能通过调控细胞周期通路抑制脑胶质瘤细胞的增殖。
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