大鼠全身振动后血清神经生长因子增加，但椎间盘退变不增加。
Increase in serum nerve growth factor but not intervertebral disc degeneration following whole-body vibration in rats.
机构信息
Department of Agricultural and Biological Engineering, Mississippi State University, 130 Creelman Street, Mississippi State, MS, 39762, USA; Center for Advanced Vehicular Systems, Mississippi State University, 200 Research Blvd, Starkville, MS 39759, USA.
Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, Mississippi State, MS 39762, USA.
出版信息
Clin Biomech (Bristol). 2022 Dec;100:105823. doi: 10.1016/j.clinbiomech.2022.105823. Epub 2022 Nov 18.
BACKGROUND
Low back pain is a leading cause of disability and is frequently associated with whole-body vibration exposure in industrial workers and military personnel. While the pathophysiological mechanisms by which whole-body vibration causes low back pain have been studied in vivo, there is little data to inform low back pain diagnosis. Using a rat model of repetitive whole-body vibration followed by recovery, our objective was to determine the effects of vibration frequency on hind paw withdrawal threshold, circulating nerve growth factor concentration, and intervertebral disc degeneration.
METHODS
Male Sprague-Dawley rats were vibrated for 30 min at an 8 Hz or 11 Hz frequency every other day for two weeks and then recovered (no vibration) for one week. Von Frey was used to determine hind paw mechanical sensitivity every two days. Serum nerve growth factor concentration was determined every four days. At the three-week endpoint, intervertebral discs were graded histologically for degeneration.
FINDINGS
The nerve growth factor concentration increased threefold in the 8 Hz group and twofold in the 11 Hz group. The nerve growth factor concentration did not return to baseline by the end of the one-week recovery period for the 8 Hz group. Nerve growth factor serum concentration did not coincide with intervertebral disc degeneration, as no differences in degeneration were observed among groups. Mechanical sensitivity generally decreased over time for all groups, suggesting a habituation (desensitization) effect.
INTERPRETATION
This study demonstrates the potential of nerve growth factor as a diagnostic biomarker for low back pain due to whole-body vibration.
背景
下背痛是导致残疾的主要原因，在工业工人和军人中，常与全身振动暴露有关。虽然全身振动引起下背痛的病理生理机制已在体内进行了研究，但关于下背痛诊断的数据却很少。本研究使用重复全身振动后恢复的大鼠模型，旨在确定振动频率对后爪撤回阈值、循环神经生长因子浓度和椎间盘退变的影响。
方法
雄性 Sprague-Dawley 大鼠每隔一天以 8Hz 或 11Hz 的频率振动 30 分钟，持续两周，然后恢复（无振动）一周。使用 Von Frey 确定每两天的后爪机械敏感性。每四天测定一次血清神经生长因子浓度。在三周终点，对椎间盘进行组织学退变分级。
结果
8Hz 组神经生长因子浓度增加了三倍，11Hz 组增加了两倍。8Hz 组在一周恢复期结束时神经生长因子浓度并未恢复到基线水平。神经生长因子血清浓度与椎间盘退变不一致，因为各组之间没有观察到退变的差异。所有组的机械敏感性通常随时间下降，这表明存在习惯化（脱敏）效应。
结论
本研究表明神经生长因子作为全身振动引起的下背痛的诊断生物标志物具有潜力。
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