Liu Shuyu, Mohri Shinsuke, Manabe Yuki, Ejima Akika, Sato Kenji, Sugawara Tatsuya
Graduate School of Agriculture, Kyoto University, Kyoto 606, Japan.
Graduate School of Agriculture, Kyoto University, Kyoto 606, Japan.
J Photochem Photobiol B. 2022 Dec;237:112601. doi: 10.1016/j.jphotobiol.2022.112601. Epub 2022 Nov 18.
Photoaging is characterized by skin dysfunction and wrinkle formation predominantly caused by chronic exposure to ultraviolet (UV) irradiation. Collagen peptides are well-recognized as nutritional supplements for enhancing skin health. Gly-Pro, a dipeptide found in collagen as a major repetitive sequence, is considered a prospect collagen peptide derivative that displays anti-photoaging potential. Herein, we evaluated the photoprotective effects of Gly-Pro in normal human dermal fibroblasts (NHDFs). Pretreatment by Gly-Pro at a concentration of 0.1 μM inhibited UVA-driven generation of reactive oxygen species (ROS) in NHDFs and attenuated UVA-induced changes in mRNA expression and activation of proteins of the MAPK-NF-κB signaling pathway. Meanwhile, Pro-Gly and cyclo(-Gly-Pro), two dipeptides that are structurally similar to Gly-Pro, depicted less anti-photoaging effects against UVA irradiation. Collectively, our data suggests that Gly-Pro has potential as a novel ingredient in nutricosmetic products for skincare and anti-photoaging.
光老化的特征是皮肤功能障碍和皱纹形成，主要由长期暴露于紫外线（UV）照射引起。胶原蛋白肽作为增强皮肤健康的营养补充剂已得到广泛认可。甘氨酸-脯氨酸（Gly-Pro）是胶原蛋白中主要的重复序列二肽，被认为是一种具有抗光老化潜力的胶原蛋白肽衍生物。在此，我们评估了甘氨酸-脯氨酸对正常人皮肤成纤维细胞（NHDFs）的光保护作用。0.1μM浓度的甘氨酸-脯氨酸预处理可抑制NHDFs中UVA驱动的活性氧（ROS）生成，并减弱UVA诱导的MAPK-NF-κB信号通路mRNA表达变化和蛋白激活。同时，与甘氨酸-脯氨酸结构相似的两种二肽，脯氨酸-甘氨酸（Pro-Gly）和环（甘氨酸-脯氨酸）[cyclo(-Gly-Pro)]，对UVA照射的抗光老化作用较小。总体而言，我们的数据表明，甘氨酸-脯氨酸有潜力成为营养化妆品中用于护肤和抗光老化的新型成分。
