Department of Psychology, University of Alberta, Edmonton T6G 2E9, Alberta, Canada.
Developmental Integrative Biology Research Group, Department of Biological Sciences, University of North Texas, Denton TX76205, USA.
Behav Brain Res. 2023 Feb 15;439:114228. doi: 10.1016/j.bbr.2022.114228. Epub 2022 Nov 24.
We know little about how - or even if in some species - fish shoal in darkness. We hypothesized that 'dark shoaling' occurs in zebrafish and therefore must depend upon lateral line sensory input. Shoaling in groups of five adult zebrafish was analyzed with motion tracking software. We measured average inter-individual distance, time near the arena wall (thigmotaxis zone) and total distance traveled under normal room light, and in near-complete darkness (infrared light at 850 nm). These observations were repeated in fish treated with cobalt chloride (CoCl), which ablates lateral line function. In untreated controls, dark shoaling was reduced compared to in light, but nonetheless still present. Elimination of lateral line sensory input by CoCl treatment similarly reduced, but did not eliminate, shoaling under both light and dark. Our findings indicate that normal zebrafish shoaling in light or dark requires both visual and lateral line inputs, with neither alone sufficient for normal shoaling.
我们对鱼类在黑暗中是如何（甚至在某些物种中是否）进行鱼群游动知之甚少。我们假设斑马鱼存在“暗鱼群游动”现象，因此它必须依赖于侧线感觉输入。使用运动跟踪软件分析了 5 条成年斑马鱼的群体游动。我们测量了个体间的平均距离、靠近竞技场墙壁的时间（触壁区）以及在正常室内光线下和近完全黑暗（850nm 红外光）下的总行进距离。在接受氯化钴（CoCl）处理的鱼中重复了这些观察，CoCl 处理会破坏侧线功能。在未处理的对照组中，与在光线下相比，黑暗中的鱼群游动减少了，但仍然存在。通过 CoCl 处理消除侧线感觉输入同样减少了，但并没有消除在光和暗条件下的鱼群游动。我们的发现表明，正常的斑马鱼在光或暗条件下的群体游动需要视觉和侧线输入，两者缺一不可，否则无法正常进行鱼群游动。
