埃弗罗霉素同系物的发现及基于异源表达对自身抗性机制的深入了解。

Discovery of Efrotomycin Congeners and Heterologous Expression-Based Insights into the Self-Resistance Mechanism.

作者信息

Fang Chunyan, Zhang Qingbo, Zhang Wenjun, Zhang Changsheng, Zhu Yiguang

机构信息

Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.

University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

J Nat Prod. 2022 Dec 23;85(12):2865-2872. doi: 10.1021/acs.jnatprod.2c00986. Epub 2022 Nov 29.

DOI:10.1021/acs.jnatprod.2c00986

PMID:36445346

Abstract

Four new efrotomycins, A1-A4 (-), were isolated from the salt mine-derived DSM 45679 and structurally determined. Efrotomycins A3 () and A4 () feature a tetrahydrofuran ring configured distinctly from known elfamycins. Heterologous expression of the efrotomycin gene cluster ( BGC) in SBT18 led to efrotomycin B1 (), the yield of which was improved several fold upon introduction of the transporter gene , a putative self-resistance determinant outside of the BGC.

摘要

从盐矿来源的DSM 45679中分离出四种新的埃弗罗霉素A1 - A4（-），并确定了其结构。埃弗罗霉素A3（）和A4（）具有一个与已知的埃尔法霉素明显不同构型的四氢呋喃环。埃弗罗霉素基因簇（BGC）在SBT18中的异源表达产生了埃弗罗霉素B1（），在引入转运蛋白基因后其产量提高了几倍，该转运蛋白基因是BGC之外一个假定的自我抗性决定因素。