Yan Li-Hua, Zhang Yu-Jie, Hu Hai-Jie, Zhang Chuan, Wang Yue, Xu Xue-Tian, Zhang Tong-Cun, Su Rui, Luo Xue-Gang
Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education & Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin, 300192, China.
Macromol Biosci. 2023 Mar;23(3):e2200173. doi: 10.1002/mabi.202200173. Epub 2023 Jan 12.
It is always big challenges for hyaluronic acid (HA) in transmembrane absorbing and efficient delivering to the skin. Pep-1, as one of the cell-penetrating peptides, has been documented to permeate various substances across cellular membranes without covalent binding. Here, a novel hyaluronic acid binding peptide (named HaBP) is designed, and then combined with Pep-1 to enhance the cell-penetrating efficiency of HA. The results of ELISA and immunofluorescence assay show that HaBP could bind with HA very well, and a combination of Pep-1 and HaBP could efficiently improve the transmembrane ability of HA. Furthermore, HA gradually enters the dermis from the surface of the skin in mice when it is administrated with both HaBP and Pep-1, while there are no obvious allergies or other adverse reactions during this process. This study finds a new method to promote the efficient transmembrane and transdermal absorption of HA, and throws some light on further research on the development of hyaluronic acid and its related cosmetics or drugs.
对于透明质酸（HA）而言，跨膜吸收并有效递送至皮肤始终是巨大挑战。穿膜肽Pep-1作为其中之一，已被证明能使各种物质在不发生共价结合的情况下穿过细胞膜。在此，设计了一种新型透明质酸结合肽（命名为HaBP），然后将其与Pep-1结合以提高HA的穿膜效率。酶联免疫吸附测定（ELISA）和免疫荧光分析结果表明，HaBP能与HA很好地结合，并且Pep-1与HaBP的组合能有效提高HA的跨膜能力。此外，当同时给予HaBP和Pep-1时，HA在小鼠体内从皮肤表面逐渐进入真皮，在此过程中未出现明显的过敏或其他不良反应。本研究找到了一种促进HA高效跨膜和透皮吸收的新方法，为透明质酸及其相关化妆品或药物的进一步研发提供了思路。
