Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China; Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Jinan University, Guangzhou 510632, China.
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China; Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Jinan University, Guangzhou 510632, China.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:323-329. doi: 10.1016/j.colsurfb.2018.08.061. Epub 2018 Aug 29.
A hyaluronic acid-containing ethosomes (HA-ES) as the transdermal drug delivery system was prepared in this work, and rhodamine B (RB) was used as a model drug to be encapsulated. The obtained HA-ES-RB was then characterized by the surface morphology, entrapment efficiency, drug loading and the stability. Results showed that the prepared HA-ES-RB was spherical and showed good dispersion as well as the stability, with a particle size of below 100 nm. The skin permeation experiments were carried out in vitro with the Franz diffusion cells and the rat dorsal skins were used. It was found that the penetration effect of HA-ES-RB was much better than that of ES-RB. The fluorescence microscopy image showed that HA-ES-RB penetrated into the deepest dermis. The excellent transdermic drug delivery effect of HA-ES-RB maybe attributed from its smaller size, hydration of hyaluronic acid as well as greater potential targeting to skin and skin appendages of liposomal carriers. Moreover, the HA-ES delivery system showed non-cytotoxicity to normal cells, indicating a good biocompatibility. This work provded a hyaluronic acid-containing ethosomes which can offer a quick, high efficient, safe and self-administered transdermal drug delivery system.
本文制备了一种含有透明质酸的醇质体（HA-ES）作为经皮给药系统，并将罗丹明 B（RB）作为模型药物进行包封。所得的 HA-ES-RB 通过表面形态、包封效率、载药量和稳定性进行了表征。结果表明，所制备的 HA-ES-RB 呈球形且具有良好的分散性和稳定性，粒径小于 100nm。通过Franz 扩散池进行了体外皮肤渗透实验，使用了大鼠背部皮肤。结果发现，HA-ES-RB 的渗透效果明显优于 ES-RB。荧光显微镜图像显示，HA-ES-RB 渗透到最深的真皮层。HA-ES-RB 具有优异的经皮给药效果，可能归因于其较小的尺寸、透明质酸的水合作用以及脂质体载体对皮肤和皮肤附属物更大的靶向潜力。此外，HA-ES 给药系统对正常细胞无细胞毒性，表明其具有良好的生物相容性。本工作提供了一种含有透明质酸的醇质体，可为快速、高效、安全的经皮给药系统提供便利。
