Family and Consumer Science Department, New Mexico State University, Las Cruces, NM, 88003, USA.
Biology Department, New Mexico State University, Las Cruces, NM, 88003, USA.
Sci Rep. 2022 Nov 30;12(1):20655. doi: 10.1038/s41598-022-25190-6.
Food associated diseases pose significant public health threat in the United States. Health risks associated with food-borne pathogens drive the need for constant monitoring of food products. An efficient method that can diagnose food-borne pathogens rapidly will be invaluable and in high demand. In this study, we showed the feasibility of a novel rapid detection platform based on fluorescence imaging/detection that combines a user-friendly, portable loop mediated isothermal amplification (LAMP) reaction device and a smartphone-based detection system. The proposed platform was used to detect Staphylococcus aureus which is one of the most important food-borne pathogen especially dairy products. The complete protocol is quicker; the reaction is performed under isothermal conditions and completed in 1 h or less. Experimental results show that LAMP assays were ten-fold more sensitive than PCR-based detection. The proposed smartphone detection system was able to detect and quantify LAMP assay samples containing three different concentrations of S. aureus from 10 CFU/mL down to 10 CFU/mL. The present proof-of-concept study demonstrated that this platform offers a portable, easy to use method for measuring target pathogens with LAMP amplification.
食源性疾病在美国对公众健康构成重大威胁。与食源性病原体相关的健康风险促使人们需要对食品进行持续监测。一种能够快速诊断食源性病原体的有效方法将具有巨大的价值并受到高度需求。在本研究中，我们展示了一种基于荧光成像/检测的新型快速检测平台的可行性，该平台结合了用户友好、便携式环介导等温扩增（LAMP）反应装置和基于智能手机的检测系统。该平台用于检测金黄色葡萄球菌，金黄色葡萄球菌是最重要的食源性病原体之一，尤其是乳制品。完整的方案更快；反应在等温条件下进行，可在 1 小时或更短的时间内完成。实验结果表明，LAMP 检测比基于 PCR 的检测灵敏十倍。所提出的智能手机检测系统能够检测和定量 LAMP 检测样品，这些样品包含三种不同浓度的金黄色葡萄球菌，浓度从 10 CFU/mL 降低到 10 CFU/mL。本概念验证研究表明，该平台为使用 LAMP 扩增测量目标病原体提供了一种便携式、易于使用的方法。
