• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

骨髓来源的巨噬细胞的自噬重编程。

Autophagic reprogramming of bone marrow-derived macrophages.

机构信息

Biotechnology Program, School of Sciences and Engineering, The American University in Cairo, 11835, Cairo, Egypt.

Children's Cancer Hospital, 57357, Cairo, Egypt.

出版信息

Immunol Res. 2023 Apr;71(2):229-246. doi: 10.1007/s12026-022-09344-2. Epub 2022 Dec 1.

DOI:10.1007/s12026-022-09344-2
PMID:36451006
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10060350/
Abstract

Macro-autophagy is a highly conserved catabolic process among eukaryotes affecting macrophages. This work studies the genetic regulatory network involving the interplay between autophagy and macrophage polarization (activation). Autophagy-related genes (Atgs) and differentially expressed genes (DEGs) of macrophage polarization (M1-M2) were predicted, and their regulatory networks constructed. Naïve (M0) mouse bone marrow-derived monocytes were differentiated into M1 and M2a. Validation of the targets of Smad1, LC3A and LC3B, Atg16L1, Atg7, IL-6, CD68, Arg-1, and Vamp7 was performed in vitro. Immunophenotyping by flow cytometry revealed three macrophage phenotypes: M0 (IL-6 + /CD68 +), M1 (IL-6 + /CD68 + /Arg-1 +), and M2a (CD68 + /Arg-1). Confocal microscopy revealed increased autophagy in both M1 and M2a and a significant increase in the pre-autophagosomes size and number. Bafilomycin A increased the expression of CD68 and Arg-1 in all cell lineages. In conclusion, our approach predicted the protein targets mediating the interplay between autophagy and macrophage polarization. We suggest that autophagy reprograms macrophage polarization via CD68, arginase 1, Atg16L1-1, and Atg16L1-3. The current findings provide a foundation for the future use of macrophages in immunotherapy of different autoimmune disorders.

摘要

自噬是真核生物中一种高度保守的分解代谢过程，影响巨噬细胞。本研究探讨了自噬与巨噬细胞极化（激活）相互作用的遗传调控网络。预测了自噬相关基因（Atgs）和巨噬细胞极化（M1-M2）的差异表达基因（DEGs），并构建了它们的调控网络。将幼稚（M0）小鼠骨髓来源的单核细胞分化为 M1 和 M2a。在体外验证了 Smad1、LC3A 和 LC3B、Atg16L1、Atg7、IL-6、CD68、Arg-1 和 Vamp7 的靶点。流式细胞术免疫表型分析显示了三种巨噬细胞表型：M0（IL-6+ / CD68+）、M1（IL-6+ / CD68+ / Arg-1+）和 M2a（CD68+ / Arg-1+）。共聚焦显微镜显示自噬在 M1 和 M2a 中均增加，并且前自噬体的大小和数量显著增加。巴弗洛霉素 A 增加了所有细胞系中 CD68 和 Arg-1 的表达。总之，我们的方法预测了介导自噬与巨噬细胞极化相互作用的蛋白靶标。我们认为自噬通过 CD68、精氨酸酶 1、Atg16L1-1 和 Atg16L1-3 重新编程巨噬细胞极化。目前的研究结果为未来利用巨噬细胞治疗不同自身免疫性疾病的免疫治疗提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/4e1f9d49ac6e/12026_2022_9344_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/90d4501298bc/12026_2022_9344_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/12c4a9c13368/12026_2022_9344_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/442d98a42c69/12026_2022_9344_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/42b5534ac1be/12026_2022_9344_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/b593489852fe/12026_2022_9344_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/72e839f076e2/12026_2022_9344_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/a9b900599de9/12026_2022_9344_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/922c9aa22e35/12026_2022_9344_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/b63f3862b437/12026_2022_9344_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/4e1f9d49ac6e/12026_2022_9344_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/90d4501298bc/12026_2022_9344_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/12c4a9c13368/12026_2022_9344_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/442d98a42c69/12026_2022_9344_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/42b5534ac1be/12026_2022_9344_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/b593489852fe/12026_2022_9344_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/72e839f076e2/12026_2022_9344_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/a9b900599de9/12026_2022_9344_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/922c9aa22e35/12026_2022_9344_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/b63f3862b437/12026_2022_9344_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/10060350/4e1f9d49ac6e/12026_2022_9344_Fig10_HTML.jpg

相似文献

1
Autophagic reprogramming of bone marrow-derived macrophages.骨髓来源的巨噬细胞的自噬重编程。
Immunol Res. 2023 Apr;71(2):229-246. doi: 10.1007/s12026-022-09344-2. Epub 2022 Dec 1.
2
Preconditioning of bone marrow-derived mesenchymal stem cells highly strengthens their potential to promote IL-6-dependent M2b polarization.骨髓间充质干细胞的预处理极大地增强了其促进 IL-6 依赖性 M2b 极化的潜力。
Stem Cell Res Ther. 2018 Oct 25;9(1):286. doi: 10.1186/s13287-018-1039-2.
3
Tumor cell-released autophagosomes (TRAPs) promote immunosuppression through induction of M2-like macrophages with increased expression of PD-L1.肿瘤细胞释放的自噬体（TRAPs）通过诱导 PD-L1 表达增加的 M2 样巨噬细胞促进免疫抑制。
J Immunother Cancer. 2018 Dec 18;6(1):151. doi: 10.1186/s40425-018-0452-5.
4
Impaired macrophage autophagy increases the immune response in obese mice by promoting proinflammatory macrophage polarization.巨噬细胞自噬受损通过促进促炎巨噬细胞极化增强肥胖小鼠的免疫反应。
Autophagy. 2015;11(2):271-84. doi: 10.1080/15548627.2015.1009787.
5
[Regulatory effect of bone marrow mesenchymal stem cells on polarization of macrophages].骨髓间充质干细胞对巨噬细胞极化的调控作用
Zhonghua Gan Zang Bing Za Zhi. 2017 Apr 20;25(4):273-278. doi: 10.3760/cma.j.issn.1007-3418.2017.04.008.
6
Interleukin 6-regulated macrophage polarization controls atherosclerosis-associated vascular intimal hyperplasia.白细胞介素 6 调控的巨噬细胞极化控制动脉粥样硬化相关血管内膜增生。
Front Immunol. 2022 Jul 27;13:952164. doi: 10.3389/fimmu.2022.952164. eCollection 2022.
7
Spermine Alleviates Acute Liver Injury by Inhibiting Liver-Resident Macrophage Pro-Inflammatory Response Through ATG5-Dependent Autophagy.精胺通过 ATG5 依赖性自噬抑制肝固有巨噬细胞的促炎反应缓解急性肝损伤。
Front Immunol. 2018 May 2;9:948. doi: 10.3389/fimmu.2018.00948. eCollection 2018.
8
Vibrio cholerae porin OmpU mediates M1-polarization of macrophages/monocytes via TLR1/TLR2 activation.霍乱弧菌孔蛋白OmpU通过激活TLR1/TLR2介导巨噬细胞/单核细胞的M1极化。
Immunobiology. 2015 Nov;220(11):1199-209. doi: 10.1016/j.imbio.2015.06.009. Epub 2015 Jun 5.
9
Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages.结直肠癌来源的细胞外囊泡对单核细胞和巨噬细胞免疫表型和细胞因子分泌谱的影响。
Cell Commun Signal. 2018 Apr 24;16(1):17. doi: 10.1186/s12964-018-0229-y.
10
Acetaminophen-Induced Rat Hepatotoxicity Based on M1/M2-Macrophage Polarization, in Possible Relation to Damage-Associated Molecular Patterns and Autophagy.基于 M1/M2 巨噬细胞极化的对乙酰氨基酚诱导的大鼠肝毒性，可能与损伤相关分子模式和自噬有关。
Int J Mol Sci. 2020 Nov 26;21(23):8998. doi: 10.3390/ijms21238998.

引用本文的文献

1
Targeting Macrophage Polarization for Reinstating Homeostasis following Tissue Damage.靶向巨噬细胞极化以恢复组织损伤后的内稳态
Int J Mol Sci. 2024 Jul 2;25(13):7278. doi: 10.3390/ijms25137278.

本文引用的文献

1
The Role of Autophagy in M2 Polarization of Macrophages Induced by Micro/Nano Topography.自噬在微纳形貌诱导的巨噬细胞 M2 极化中的作用。
Int J Nanomedicine. 2020 Oct 9;15:7763-7774. doi: 10.2147/IJN.S270100. eCollection 2020.
2
Phagocytosis: Our Current Understanding of a Universal Biological Process.吞噬作用：对普遍生物学过程的现有理解。
Front Immunol. 2020 Jun 2;11:1066. doi: 10.3389/fimmu.2020.01066. eCollection 2020.
3
An evolutionary recent IFN/IL-6/CEBP axis is linked to monocyte expansion and tuberculosis severity in humans.
一个进化上较新的 IFN/IL-6/CEBP 轴与人类单核细胞扩增和结核病严重程度相关。
Elife. 2019 Oct 22;8:e47013. doi: 10.7554/eLife.47013.
4
Cytotoxic Granule Exocytosis From Human Cytotoxic T Lymphocytes Is Mediated by VAMP7.人细胞毒性 T 淋巴细胞的细胞毒性颗粒胞吐作用由 VAMP7 介导。
Front Immunol. 2019 Aug 7;10:1855. doi: 10.3389/fimmu.2019.01855. eCollection 2019.
5
New insights into autophagy in hepatocellular carcinoma: mechanisms and therapeutic strategies.肝细胞癌自噬的新见解：机制与治疗策略
Am J Cancer Res. 2019 Jul 1;9(7):1329-1353. eCollection 2019.
6
Prognostic significance of CD68 and CD163 tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis.头颈部鳞状细胞癌中 CD68 和 CD163 肿瘤相关巨噬细胞的预后意义：系统评价和荟萃分析。
Oral Oncol. 2019 Jun;93:66-75. doi: 10.1016/j.oraloncology.2019.04.019. Epub 2019 Apr 28.
7
NetworkAnalyst 3.0: a visual analytics platform for comprehensive gene expression profiling and meta-analysis.NetworkAnalyst 3.0：一个用于全面基因表达谱分析和荟萃分析的可视化分析平台。
Nucleic Acids Res. 2019 Jul 2;47(W1):W234-W241. doi: 10.1093/nar/gkz240.
8
Lipopolysaccharide mediates time-dependent macrophage M1/M2 polarization through the Tim-3/Galectin-9 signalling pathway.脂多糖通过 Tim-3/Galectin-9 信号通路介导时间依赖性巨噬细胞 M1/M2 极化。
Exp Cell Res. 2019 Mar 15;376(2):124-132. doi: 10.1016/j.yexcr.2019.02.007. Epub 2019 Feb 11.
9
In-Depth Characterization of Monocyte-Derived Macrophages using a Mass Cytometry-Based Phagocytosis Assay.基于液质联用技术的吞噬作用分析对单核细胞来源的巨噬细胞进行深入表征。
Sci Rep. 2019 Feb 13;9(1):1925. doi: 10.1038/s41598-018-38127-9.
10
IL-10-secreting human MSCs generated by TALEN gene editing ameliorate liver fibrosis through enhanced anti-fibrotic activity.TALEN 基因编辑产生的分泌 IL-10 的人骨髓间充质干细胞通过增强抗纤维化活性改善肝纤维化。
Biomater Sci. 2019 Feb 26;7(3):1078-1087. doi: 10.1039/c8bm01347k.